Identification of InDel Locus of ITGAD Gene Based on Transcriptome Sequencing and Its Association with Goat (Capra hircus) Growth Traits
WU Xian-Feng1, WANG Jin-Bao2, LIU Yuan1, CHI Chun-Mei2, HUANG Qin-Lou1, LI Wen-Yang1,*
1 Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, Fuzhou 350000, China; 2 Ningde Institute of Agricultural Science, Fuan 355017, China
Abstract：β2 integrins αD (ITGAD) is a subunit of heterodimeric surface receptor, and plays a critical role in the regulation of lipid metabolism and blood glucose balance. This work aimed to detect the candidate molecular markers for marker-assisted selection (MAS) in goats (Capra hircus).In this study, the Insertion/Deletion (InDel) polymorphism of ITGAD gene in 3 goat breeds (355 in total) was identified through DNA pooling technology and high-resolution melting (HRM) analysis . The results showed that a novel InDel locus (NC_030832.1: g.15243_15244 ins C) was detected. Furthermore, in 3 goat breeds, the polymorphic information content (PIC) of the InDel locus was 0.289~0.369. The InDel locus was consistent with Hardy-Weinberg in 3 experimental populations. Association analysis showed that this InDel locus was significantly associated with the huckle bone width of Fuqing goat and the body height, body length, and chest circumference of Jianyang Daer goat (P<0.05). It was also extremely significantly correlated with chest depth, chest width, cannon circumference, and cannon circumference index of Jianyang Daer goat (P<0.01). Moreover, the II genotype conferred positive effects on growth traits compared with the DD genotype. The present study found the significant association of ITGAD InDel locus with the growth traits in goats, which could be adopted as the molecular genetic marker for growth traits of goats.
吴贤锋, 王金宝, 刘远, 池春梅, 黄勤楼, 李文杨. 基于转录组测序的ITGAD基因InDel位点鉴定及其与山羊生长性状的关联性分析[J]. 农业生物技术学报, 2022, 30(9): 1763-1770.
WU Xian-Feng, WANG Jin-Bao, LIU Yuan, CHI Chun-Mei, HUANG Qin-Lou, LI Wen-Yang. Identification of InDel Locus of ITGAD Gene Based on Transcriptome Sequencing and Its Association with Goat (Capra hircus) Growth Traits. 农业生物技术学报, 2022, 30(9): 1763-1770.
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