Identification of JAZ Gene Family in Cajanus cajan and Expression Analysis in Response to Pathogenic Fungus Cc1-1
LI Na1, SONG Zhi -Hua1, FAN Yu-Xin1, DONG Bi-Ying1, CAO Hong-Yan1, DU Ting-Ting1, LIU Teng-Yue1, YANG Wan-Long1, YANG Qing1,2, MENG Dong1,2,*, FU Yu-Jie1,2,*
1 College of Forestry, Beijing Forestry University, Beijing 100083, China; 2 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China
Abstract：Jasmonates play a key role in plant response to biological stresses, and jasmonate ZIM-domain (JAZ) is a critical factor in jasmonic acid (JA) signal pathway. The present study screened out 24 members of JAZ family in pigeonpea (Cajanus cajan) genome, and explored phylogenetic analysis, gene chromosome mapping and protein conserved domain analysis. According to previous transcriptome data, the expression patterns of JAZs in roots and leaves were analyzed, and the expression of JAZs in leaves in response to pathogenic fungus Cc1-1 was detected by reverse transcription-PCR (RT-PCR). The results showed that there were 129~376 amino acid residues in JAZ family, and the isoelectric points were 5.94~9.37. JAZ family members could be divided into 5 subfamilies by MEGA software, and they were unevenly distributed on 6 chromosomes. The subcellular localization prediction showed that CcJAZ12 and CcJAZ18 were located in the chloroplast and CcJAZ19 in the cytoskeletal, and all the other 21 JAZs were located in the nucleus. The prediction results of cis-acting elements in the promoters showed that JAZ family genes had certain amount of JA responsive elements, and a large number of light responsive elements and a small number of abscisic acid, salicylic acid and other hormone regulatory elements. Transcriptome analysis showed that the expression of JAZs in leaves was higher than that in roots, and the expression of CcJAZ19 was the highest, which might play a more important role in the response of leaves to biological stresses. After infection of pigeonpea leaves by pathogenic fungus Cc1-1, the gene expression of JAZs in the leaves increased gradually with the infection time, and the expression of CcJAZ8, CcJAZ19, and CcJAZ23 was significantly up-regulated. The expression level of CcJAZ19 was up-regulated to 2.5 times (P<0.05) at 6 h after infection, indicating that it might participate in the defense response of JA signal to fungi. The present study provides basic data for the follow-up study on molecular regulation mechanism of JA signal.
李娜, 宋治华, 范雨欣, 董碧莹, 曹红燕, 杜婷婷, 刘腾跃, 杨琬珑, 杨清, 孟冬, 付玉杰. 木豆JAZ基因家族鉴定及其响应致病真菌Cc1-1侵染的表达分析[J]. 农业生物技术学报, 2021, 29(8): 1495-1505.
LI Na, SONG Zhi -Hua, FAN Yu-Xin, DONG Bi-Ying, CAO Hong-Yan, DU Ting-Ting, LIU Teng-Yue, YANG Wan-Long, YANG Qing, MENG Dong, FU Yu-Jie. Identification of JAZ Gene Family in Cajanus cajan and Expression Analysis in Response to Pathogenic Fungus Cc1-1. 农业生物技术学报, 2021, 29(8): 1495-1505.
[1] 陈金焕, 田玉如, 李艾佳, 等. 2020. 茉莉酸信号及其在木本植物中的研究进展[J]. 中国科学: 生命科学, 50(02): 215-226. (Chen J H, Tian Y R, Li A J, et al.2020. Jasmonic acid signaling and its research progress in woody plants[J]. Scientia Sinica Vitae, 50(02): 215-226.) [2] 黄建丽, 邓肃霜, 沈甲诚, 等. 2019. 大豆JAZ基因家族的鉴定及其对疫霉胁迫的响应[J]. 大豆科学, 38(06): 868-878. (Huang J L, Deng S S, Shen J C, et al.2019. Identification and response to Phytophthora sojae infections of JAZ gene family in soybean[J]. Soybean Science, 38(06): 868-878.) [3] 纪中华, 杨艳鲜, 拜得珍, 等. 2007. 木豆在干热河谷退化山地的生态适应性研究[J]. 干旱地区农业研究, 25(03): 158-162. (Ji Z H, Yang Y X, Bai D Z, et al.2007. The study on the ecological adaptability of Cajanus cajan (L.) Millspaugh in the degraded mountain land of dry-hot valley[J]. Agricultural Research in the Arid Areas, 25(03): 158-162.) [4] 蒋科技, 皮妍, 侯嵘, 等. 2010. 植物内源茉莉酸类物质的生物合成途径及其生物学意义[J]. 植物学报, 45(02): 137-148. (Jiang K J, Pi Y, Hou R, et al.2010. Jasmonate biosynthetic pathway: Its physiological role and potential application in plant secondary metabolic engineering[J]. Chinese Bulletin of Botany, 5(02): 137-148.) [5] 金微微, 王炎, 张会慧, 等. 2011. 茉莉酸甲酯对干旱及复水条件下烤烟幼苗叶绿素荧光特性的影响[J]. 应用生态学报, 22(12): 3157-3162. (Jin W W, Wang Y, Zhang H H, et al.2011. Effects of foliar spraying methyl jasmonate on leaf chlorophyll fluorescence characteristics of flue-cured tobacco seedlings under drought and re-waterin[J]. Chinese Journal of Applied Ecology, 22(12): 3157-3162.) [6] 宋云, 李林宣, 卓凤萍, 等. 2015. 茉莉酸信号传导在植物抗逆性方面研究进展[J]. 中国农业科技导报, 17(02): 17-24. (Song Y, Li L X, Zhuo F P, et al.2015. Progress on jasmonic acid signaling in plant stress resistant[J]. Journal of Agricultural Science and Technology, 17(02): 17-24.) [7] 孙程, 周晓今, 陈茹梅, 等. 2014. 植物JAZ蛋白的功能概述[J]. 生物技术通报, (06): 1-8. (Sun C, Zhou X J, Chen R M, et al. 2014. Comprehensive overview of JAZ proteins in plants[J]. Biotechnology Bulletin, (06): 1-8.) [8] 向妙莲, 付永琦, 何永明, 等. 2014 .茉莉酸甲酯浸种对水稻幼苗白叶枯病抗性及抗氧化酶活性的影响[J]. 中国水稻科学, 28(04): 419-426. (Xiang M L, Fu Y Q, He Y M, et al.2014. Effects of seed soaking with methyl jasmonate on resistance to bacterial leaf blight and antioxidant enzyme activities of rice seedlings[J]. Chinese Journal of Rice Science, 28(04): 419-426.) [9] 朱香. 2018. Epicoccum sorghinum的分离及多基因转玉米株系的抗性鉴定[D]. 硕士学位论文, 华中农业大学, 导师: 董五辈, pp. 1-5. (Zhu X.2018. Isolation of Epicoccum sorghinum and resistance identification of multi-transgenes maize lines[D]. Thesis for M.S., Huazhong Agricultural University, Suppervisor: Dong W B, pp. 1-5.) [10] Antico C J, Colon C, Banks T, et al.2012. Insights into the role of jasmonic acid-mediated defenses against necrotrophic and biotrophic fungal pathogens[J]. Frontiers in Biology, 7(001): 48-56. [11] Avdiushko S A, Brown G C, Dahlman D L, et al.1997. Methyl jasmonate exposure induces insect resistance in cabbage and tobacco[J]. Environmental Entomology, 26(3): 642-654. [12] Bai Y, Meng Y, Huang D, et al.2011. Origin and evolutionary analysis of the plant-specific TIFY transcription factor family[J]. Genomics, 98(2): 128-136. [13] Chao J, Zhao Y, Jin J, et al.2019. Genome-wide identification and characterization of the JAZ gene family in rubber tree (Hevea brasiliensis)[J]. Frontiers in Genetics, 10: 372. [14] Dombrowski J E.2003. Salt stress activation of wound-related genes in tomato plants[J]. Plant Physiology, 132(4): 2098-2107. [15] Kachroo A, Kachroo P.2009. Fatty acid-derived signals in plant defense[J]. Annual Review of Phytopathology, 47: 153-176. [16] Li W, Xia X, Han L, et al.2017. Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)[J]. Scientific Reports, 7(1): 1-14 [17] Melotto M, Mecey C, Niu Y, et al.2008. A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine and jasmonoyl isoleucine dependent interactions with the COI1 F-box protein[J]. The Plant Journal, 55(6): 979-988. [18] Patt J M, Robbins P S, Niedz R, et al.2018. Exogenous application of the plant signalers methyl jasmonate and salicylic acid induces changes in volatile emissions from citrus foliage and influences the aggregation behavior of Asian citrus psyllid (Diaphorina citri), vector of Huanglongbing[J]. PLOS ONE, 13(3): e193724. [19] Pauwels L, Goossens A.2011. The JAZ proteins: A crucial interface in the jasmonate signaling cascade[J]. The Plant Cell, 23(9): 3089-3100. [20] Sheard L B, Tan X, Mao H, et al.2010. Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor[J]. Nature, 468(7322): 400-405. [21] Shrestha G, Reddy G V.2019. Field efficacy of insect pathogen, botanical, and jasmonic acid for the management of wheat midge Sitodiplosis mosellana and the impact on adult parasitoid Macroglenes penetrans populations in spring wheat[J]. Insect Science, 26(3): 523-535. [22] Sobhy I S, Woodcock C M, Powers S J, et al.2017. Cis-jasmone elicits aphid-induced stress signaling in potatoes[J]. Journal of Chemical Ecology, 43(1): 39-52. [23] Thines B, Katsir L, Melotto M, et al.2007. JAZ repressor proteins are targets of the SCF COI1 complex during jasmonate signaling[J]. Nature, 448(7154): 661-665. [24] Wang Y, Pan F, Chen D, et al.2017. Genome-wide identification and analysis of the Populus trichocarpa TIFY gene family[J]. Plant Physiology and Biochemistry, 115: 360-371. [25] Wasternack C, Hause B.2013. Jasmonates: Biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany[J]. Annals of Botany, 111(6): 1021-1058. [26] Ye H, Du H, Tang N, et al.2009. Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice[J]. Plant Molecular Biology, 71(3): 291-305.
