Abstract：Fusarium wilt and Anthracnose are the most common and major diseases in the strawberry (Fragaria ananassa), and the pathogens are Fusarium oxysporum and Colletotrichum spp., respectively. At present, the main detection methods are traditional pathogen culture method and conventional PCR method, but loop-mediated isothermal amplification (LAMP) can be used for rapid detection of Fusarium wilt and Anthracnose in strawberry. In this study, a set of LAMP amplification primers was designed for the conserved region of intergenic spacer (IGS) in rDNA of Fusarium oxysporum, and the LAMP specific primers were designed to detect the specific sequence of internal transcribed spacer (ITS) in Colletotrichum spp. The LAMP technique was used to identify and detect strawberry Fusarium oxysporum and Colletotrichum spp. rapidly and accurately. The results showed that the established LAMP method was able to specifically detect F. oxysporum and Colletotrichum spp., and the detection sensitivity was 10×10-5 ng/uL, which was 1 000 times that of conventional PCR. As a result, the rapid LAMP detection method of Fusarium wilt and Anthracnose in strawberry developed in this study had the advantages of high sensitivity, rapidity and simplicity, which could be popularized to grass-roots units and play an important role in real-time monitoring and early warning of strawberry pathogens.
史芳芳, 王雷. 草莓枯萎病和炭疽病的双重LAMP快速病原鉴定[J]. 农业生物技术学报, 2021, 29(6): 1215-1221.
SHI Fang-Fang, WANG Lei. Rapid Pathogen Identification of Fusarium Wilt and Anthracnose in Strawberry (Fragaria ananassa) by Double LAMP. 农业生物技术学报, 2021, 29(6): 1215-1221.
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