联系我们 加入收藏
 
年期检索 高级检索
33
2025年8月3日 星期日
  2017, Vol. 25 Issue (2): 335-344    
  研究资源与技术改进 本期目录 | 过刊浏览 | 高级检索 |
环介导等温扩增法检测6种转基因大豆
周杰1,黄文胜2,邓婷婷2,吴亚君2,胡秋辉3,陈颖4
1. 中国检验检疫科学研究院农产品安全研究中心
2. 中国检验检疫科学研究院 农产品安全研究中心
3. 南京财经大学 食品科学与工程学院
4. 中国检验检疫科学研究院
Detection of Six Kinds of Genetically Modified Soybean (Glycine max) by LAMP Method
, , , , ,ying chen
全文: PDF (8235 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 摘 要 随着全球商品化生产的转基因产品日益增多,转基因品系检测已逐渐成为国内及国际上转基因成分检测的发展趋势。为实施我国转基因农产品的进出境、环境释放及标签标识等相关法规,实现进口转基因大豆(Glycine max)的有效管理,本研究针对DAS-81419-2、DP356043-5、A2704-12、FG72、BPS-CV127-9和MON89788等6种常见转基因大豆的旁侧序列,分别设计了环介导等温扩增(loop-mediated isothermal amplification, LAMP)引物。通过引物筛选及方法的特异性、灵敏度及检出限验证,建立了转基因大豆DAS-81419-2、DP-356043、A2704-12、FG72、BPS-CV127-9和MON89788的LAMP检测方法。结果表明,所建立的方法能特异、稳定地检测转基因大豆DAS-81419-2、DP356043-5、A2704-12、FG72、BPS-CV127-9和MON89788,检测灵敏度可达到0.1%(W/W),通过在反应终产物中加入SYBR GreenⅠ染料,用肉眼即可直接判断检测结果,检测时间在30 min以内。本方法快速准确,且不需要复杂昂贵的仪器设备,适用于检测机构及相关执法部门对转基因产品的快速检测。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
周杰
黄文胜
邓婷婷
吴亚君
胡秋辉
陈颖
关键词 转基因大豆品系环介导等温扩增(LAMP)特异性灵敏度检出限准确性    
Abstract:Abstract With the increasing of the global commercial production of genetically modified products, the detection of transgenic lines has gradually become the development trend of the detection of genetically modified components in internal and international. To carry out the regulations of genetically modified organisms in China's inward and outward, environmental release and labeling, and achieve effective management of genetically modified soybean (Glycine max), the loop-mediated isothermal amplification (LAMP) primers were designed according to the flanking sequence of genetically modified soybean DAS-81419-2, DP356043-5, A2704-12, FG72, BPS-CV127-9 and MON89788, separately. Through specificity, sensitivity and limit of detection testing, an LAMP method for the inspection of genetically modified soybean DAS-81419-2, DP356043-5, A2704-12, FG72, BPS-CV127-9 and MON89788 was developed. The results showed that this method could detect genetically modified soybean DAS-81419-2, DP356043-5, A2704-12, FG72, BPS-CV127-9 and MON89788 specifically and stably with a low detection limit of 0.1% (W/W). Since the LAMP detection could be finished within 30 min and the results could be directly detected according to the color change produced by SYBR Green Ⅰ dye with the naked eye. This method allowed rapid and accurate detection of genetically modified products on the inspection agency and relevant law enforcement departments, and non-essential of complex and expensive equipment.
Key wordsGenetically modified soybean    Loop-mediated isothermal amplification (LAMP)    Specificity detection    Sensitivity detection    Limit of detection    Accuracy
收稿日期: 2016-08-03      出版日期: 2017-01-13
通讯作者: 陈颖     E-mail: chenyingcaiq@163.com
引用本文:   
周杰 黄文胜 邓婷婷 吴亚君 胡秋辉 陈颖. 环介导等温扩增法检测6种转基因大豆[J]. , 2017, 25(2): 335-344.
ying chen. Detection of Six Kinds of Genetically Modified Soybean (Glycine max) by LAMP Method. , 2017, 25(2): 335-344.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2017/V25/I2/335
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn