Cloning of Yak (Bos grunniens) CAV1 Gene and Its Expression in Major Organs of Female Reproductive System
ZHANG Rui, WANG Jing-Lei, PAN Yang-Yang, WANG Jing-Yu, HU Xue-Quan, MA Rui, CUI Yan, YU Si-Jiu*
College of Veterinary Medicine/Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Caveolin-1 (Caveolin-1, CAV1) is a membrane protein associated with endocytosis, extracellular matrix organization, cholesterol distribution, cell migration and signaling.The aim of the present study was to explore the expression and biological function of CAV1 in the reproductive system of female yak (Bos grunniens) under normal physiological conditions. In this study, the uterus, ovaries and fallopian tubes of female yaks at different reproductive stages (follicula, luteal and gestation) were used as materials. The mRNA and protein expression of CAV1 were analyzed by gene cloning, qRT-PCR, Western blot and immunohistochemistry (IHC). The results showed that the CAV1 gene (GenBank No. MN127963) was highly conserved. The CAVI gene sequence of yak was different from ordinary cattle (Bos taurus), but the encoded amino acid was constant. The CAVI gene of yak had the closest relatives to Bos taurus and Ovis aries, and the farthest relatives to Felis catus and Equus saddle. and the encoded protein was a stable hydrophobic membrane protein. CAV1 was expressed in the main organs of the female yak reproductive system, while the expression level of CAV1 protein in the oviduct, ovary and uterus in the follicular phase was significantly higher than that in the luteal phase and gestational period (P<0.05). The expression level of CAV1 protein in the fallopian tube of the gestational period was significantly higher than that in the luteal phase (P<0.05). The expression level of CAV1 protein in the ovary and uterus of the luteal phase was significantly higher than that in the gestation phase(P<0.05). Immunohistochemistry results showed that CAV1 is expressed in the oviduct mucosa epithelium, serous gland, ovarian follicle membrane, follicular granule layer, luteal cells, reproductive epithelium, endometrium and uterine gland. This study provides a theoretical basis for further exploration of CAV1 involved in mammalian reproductive physiology regulation, which is helpful to the study of the breeding rules of mammals in the plateau.
张瑞, 王靖雷, 潘阳阳, 王静瑜, 胡学权, 马睿, 崔燕, 余四九. 牦牛CAV1基因克隆及其在雌性生殖系统主要器官中的表达定位[J]. 农业生物技术学报, 2020, 28(4): 681-692.
ZHANG Rui, WANG Jing-Lei, PAN Yang-Yang, WANG Jing-Yu, HU Xue-Quan, MA Rui, CUI Yan, YU Si-Jiu. Cloning of Yak (Bos grunniens) CAV1 Gene and Its Expression in Major Organs of Female Reproductive System. 农业生物技术学报, 2020, 28(4): 681-692.
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