Expression and Distribution of CRY1 in the Reproductive Axis of Male Yak (Bos grunniens)
CHEN Jian-Nan1, WANG Qi2, WANG Yuan-Yuan2, LV Chen2, ZHAO Cai-Ying1, ZHANG Yong1,2, MA You-Ji3, ZHANG Quan-Wei1,*, ZHAO Xing-Xu1,2,*
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 3 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Cryptochrome 1 (CRY1) gene is a member of mammalian clock genes. Its expressed protein is both a photoreceptor and a part of the circadian clock, which could be delivered to the effector via the humoral and neural pathways to regulate mammalian physiology, biochemistry and behavior. In this study, the samples (the hypothalamus, pituitary, pineal and testicular tissues) of yak were collected, and the sequence of CRY1 coding region was cloned and the expression of CRY1 in adult male yak reproductive axis and yak testes of different ages (30 d, 2, 4 and 6 years old) was analyzed by qRT-PCR (real-time fluorescent quantitative PCR), Western blot and immunohistochemical staining. The results showed that the CRY1 gene CDS was 1 712 bp in length and the ORF was 1 257 bp (GenBank No. MN460656), encoding a total of 418 amino acid residues, without transmembrane domain, and the encoded protein was a soluble protein. Tissue expression and localization revealed that CRY1 mRNA and protein were expressed in the hypothalamus, pituitary, testis and pineal tissues of yak, and the expression level in pineal tissue was significantly higher than other tissues (P<0.01). Immunohistochemical staining showed positive expression in the hypothalamic paraventricular nucleus, pituitary cells, testicular spermatogenic cells, pineal cells and glial cells. The expression of CRY1 mRNA and protein in the testis of 30 d was the lowest in the testis tissues of different age groups, and the expression level gradually increased with age. The results showed that the CRY1 gene was relatively more conservative in animal evolution and was expressed in the male yak gland axis and the yak testes of different ages, suggesting that it might play an important role in the reproductive process of yak. The above results provides the basis for the biological function of the yak biorhythm gene CRY1 and its related research on the regulation of yak reproductive physiology.
陈健楠, 王琪, 王媛媛, 吕琛, 赵彩英, 张勇, 马友记, 张全伟, 赵兴绪. CRY1在雄性牦牛生殖轴中的表达与分布[J]. 农业生物技术学报, 2019, 27(10): 1869-1877.
CHEN Jian-Nan, WANG Qi, WANG Yuan-Yuan, LV Chen, ZHAO Cai-Ying, ZHANG Yong, MA You-Ji, ZHANG Quan-Wei, ZHAO Xing-Xu. Expression and Distribution of CRY1 in the Reproductive Axis of Male Yak (Bos grunniens). 农业生物技术学报, 2019, 27(10): 1869-1877.
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