Tissue Distribution of GAS6 Gene and Its Developmental Expression in Fat Tissue of Jinmao Black Chicken (Gallus gallus domesticus)
CHEN Lan, ZHANG Tao, WANG Jin-Yu, YIN Xue-Mei, CHEN Fu-Xiang, ZHANG Gen-Xi, WEI Xi-Hui, MAO Jing
1 College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2 Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou 225009, China; 3 Jiangsu Sandeli Animal Husbandry Development Co., Ltd., Changzhou 213200, China
Abstract：Growth arrest-specific gene 6 (GAS6) is differentially expressed during the differentiation of chicken preadipocytes and may be involved in regulating the fat deposition of chicken (Gallus gallus domesticus). However, its tissue distribution and developmental expression pattern in chicken is yet unknown. To study the tissue distribution and developmental expression profiles of GAS6 gene, six 4-week old Jinmao Black chickens (3 males and 3 females) were used. qRT-PCR was used to detect the tissue distribution of chicken GAS6 genes in 8 tissues (heart, liver, spleen, lung, kidney, breast muscle, leg muscle and abdominal fat). The developmental expression pattern of GAS6 gene in chicken abdominal fat was detected at different growth stages (1-day, 4, 8, 12, 16-week old). The results showed that GAS6 gene was highly expressed in abdominal fat and moderately expressed in lung and kidney of male and female chickens. The expression pattern of GAS6 gene in the abdominal fat showed trends of “M” and “W” type at different developmental stages of male and female chickens. The expression peak appeared before 4-week old both in male and female chickens. This current study combined with high-throughput sequencing results showed that GAS6 gene might influence the early development of abdominal fat by regulating the proliferation and growth arrest of adipocytes in chicken. This study revealed the tissue expression profiles of GAS6 gene in Jinmao Black chickens and analyzed its developmental expression pattern in abdominal fat, which provides theoretical basis for the further study on the function and expression regulation mechanism of GAS6 gene in chicken fat deposition.
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