Abstract：Myogenin gene (MyoG) is a member of myogenic regulatory factors (MRFs) family and plays an important role in regelation of muscle building. To explore the expression profile and expression pattern of MyoG gene and its possible mechanism of action, a pair of fluorescence quantitative primers according to the cloned coding sequence (CDS) of MyoG in Jinghai yellow chicken (Gallus gallus) was designed. Real-time fluorescent quantitative PCR was used to detect the expression level of MyoG in different tissues to draw the expression profile. Then, the expression levels of MyoG gene in high expression tissue of breast and leg muscle at four time points were detected to study the expression pattern. The results showed that MyoG mainly expressed in breast and leg muscle and the expression level was extremely significant higher (P＜0.01) than that in other tissues. Expression of MyoG was also detected in heart and ovary, however, the expression level was very low. Expression pattern of MyoG showed a change trend. The variation of MyoG expression in breast and leg muscle of female chicken was stable. The expression in breast and leg muscle of male chicken showed significant changes. Expression of MyoG gene achieved the highest level at 4 weeks of age and lowest level at 0 week of age. For male and female chicken, expression level of MyoG gene in breast muscle was higher than that in leg muscle. This study revealed the expression profile and expression pattern with the growth and development of muscle of Jinghai yellow chicken, and might lay a foundation for further study on mechanism of action and expression regulation of MyoG gene.
Francetic T, Li Q, 2011. Skeletal MyoGenesis and Myf5 activation.Transcription, 2(3):109-114. Rescan P Y, 2001. Regulation and functions of MyoGenic regulatory factors in lower vertebrates. Comp Biochem Physiol B, 130 (1):1-12. Genxi Z, Ying T, Tao, Jinyu W, Yongjuan W, 2014. Expression profiles and association analysis with growth traits of the MyoG and Myf5 genes in the Jinghai yellow chicken. Molecular biology reports, 1-8. Gregory D J, Waldbieser G C and Bosworth B G, 2004. Cloning and characterization of MyoGenic regulatory genes in three Ictalurid species. Anim genet, 35(6):425-430. Hasty P, Bradley A, Morris J H, Edmondson D E, Venuti J M, Olson E N and Klein W H, 1993. Muscle deficiency and neonatal death in mice with a targeted mutation in the MyoGenin gene. Nature, 364(6437):501–506 Jia J, Liu H H, Si J M, Han C C, Huang K L, Li L and Wang J W, 2010. Cloning of duck MyoG gene coding domain sequence and its developmental expression in duck heart tissuues. Chinese Journal of Animal Science, 46(21): 1-4（贾径，刘贺贺，司建明，韩春春，黄锎靓，李亮，王继文，2010. 鸭MyoG基因编码区的克隆及其在鸭心肌组织发育中的表达研究. 中国畜牧杂志, 46(21): 1-4.） JiuSheng W., YueHuan L and NingYing X. 2009 Histological characteristics of musculus longissimus dorsi and their correlation with restriction fragment length polymorphism of the MyoGenin gene in Jinghua× Pietrain F2 crossbred pigs. Meat Science 81(1), 108-115. Liu H H, Wang J W, Li L, et al. Molecular evolutionary analysis of the duck MYOD gene family and its differential expression pattern in breast muscle development[J].Br Poult Sci,2011,52(4):423-31. Ma H M, Liu X C, Shi Q S et al, 2004.Tissue expression profile of MyoG gene in pigs. Research Advance of Animal biotecenology, 9(2):221-223.（马海明,刘小春,施启顺,等.猪肌细胞生成素基因的组织表达谱分析.动物生物技术研究进展,9(2):221-223.） Megeney LA, Rudinick MA (1995) Determination versus differentiation and the MyoD family of transcription factors. Biochem Cell Biol 73(9–10):723–732 Nabeshima Y, Hanaoka K, Hayasaka M, Esumi E, Li S, Nonaka I, Nabeshima Y (1993) MyoGenin gene disruption results in perinatal lethality because of severe muscle defect. Nature 364:532–535 Naidu PS, Ludolph DC, To RQ, Hinterberger TJ, Konieczny SF (1995) MyoGenin and MEF2 function synergistically to activate the MRF4 promoter during MyoGenesis. Mol Cell Biol 15(5):2707–2718 Olson EN (1990) MyoD family: a paradigm for development. Genes Dev 4:1454–1461 Ropka-Molik K., Eekert R and Piorkowska K. 2011 The expression pattern of MyoGenic regulatory factors MyoD, Myf6 and Pax7 in postnatal porcine skeletal muselea. Gene Expression Patterns 11, 79-83 Sun W, Wang P, Ding J T, Ma Y H, Guang W J, Chu M X, Li B C, Wu W Z, Chen L, 2010. Developmental changes of gene expression of Myostain and MyoGenin genes and their association analysis with carcass traits in hu sheep. Scientia Agricultura Sinica, 43(24): 5129-5136（孙伟，王鹏，丁家桐，马月辉，关伟军，储明星，李碧春，吴文忠，陈玲，2010.湖羊Myostain和MyoGenin基因表达的发育性变化及屠宰性状的关联分析.中国农业科学,43(24): 5129-5136.） Wright W E, Sasson D A, Lin V K, et al. a factor regulating the MyoGenesis,has a domain homologous to MyoD[J].Cell,1989,56(4):607-617. Xue M., Zan L. S., Gao L and Wang H. B. 2011 A novel polymorphism of the MyoGenin gene is associated with body measurement traits in native Chinese breeds. Genet. Mol. Res. 10(4), 2721-2728. Yang X J, Chen J, Xu Q F, Wei X H Zhao R Q, 2006. Developmental changes of Myostain and MyoGenin genes expression in longissimus dorsi muscle of Erhualian and Large White pigs. Journal of Nanjing Agricultural University, 29(3): 64-68.（杨晓静，陈 杰，胥清富，韦习会，赵茹茜，2006. 二花脸猪和大白猪背最长肌中肌肉生长抑制素和生肌调节因子基因的表达及其性别特点. 南京农业大学学报, 29(3): 64-68.） Yang X J, Chen J, Xu Q F, Wei X H, Zhao R Q. Developmental changes of myostatin and MyoGenin genes expression in longissimus dorsi muscle of Erhualian and Large White pigs. Journal of Nanjing Agricultural University, 2006, 29(3): 64-68. (in Chinese) Yin H. D., Zhan, Z. C., Lan X., Zhao X. L., Wang Y and Zhu Q. 2011 Association of MyF5, MyF6 and MyoG gene polymorphisms with carcass traits in Chinese Meat Type Quality chicken populations. J. Anim. Vet. Adv. 10, 704-708. Zhang G X, 2010. Studies on genetic diversity and genetic effect of the Myostatin gene on growth and reproductive traits of Bian chicken. Dissertatio for Ph. D., Yangzhou University, supervisor: Wang J Y, pp.115-116（张根喜，2010.边鸡遗传多样性及Moystatin 基因对生长和繁殖性状的遗传效应研究，博士学位论文.扬州大学，导师：王金玉，PP：115-116）
