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| Analysis on the Promotion of Root Growth and Drought Resistance Mechanism of Understory Economic Plants by Kaempferol |
| CHEN Yang1,2,*, ZHU Tian-Tian1,2,*, SUPRUN Ivan Ivanovich4, FU Yu-Jie1,2, YANG Qing1,2, DONG Bi-Ying1,3,**, MENG Dong1,2,** |
1 State Key Laboratory of Forest Resources High Efficiency Production, Beijing Forestry University, Beijing 100083, China;
2 College of Forestry, Beijing Forestry University, Beijing 100083, China;
3 School of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China;
4 North Caucasian Federal Scientific Center of Horticulture, Viticulture, Winemaking, Krasnodar 350901, Russia |
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Abstract Elucidating the regulatory mechanisms of plant secondary metabolites and their associated genes under drought stress is fundamental for enhancing crop resistance and improving yield. Kaempferol is a flavonoid compound widely present in plants, and the mechanisms by which it promotes plant growth and development as well as induces plant stress resistance remain to be studied. This study conducted differential metabolite analysis on the rhizosphere metabolites of Scutellaria baicalensis under monoculture and intercropping conditions, and performed transcriptome sequencing on S. baicalensis samples, the results showed that the growth performance of S. baicalensis under intercropping was significantly higher than that under monocropping. Metabolomic profiling identified significantly elevated levels of kaempferol as a key differential secondary metabolite. Transcriptomic analysis further pinpointed the microtubule-associated protein WVD2-like 4 as markedly upregulated. Tissue-specific expression analysis revealed that WVD2-like 4 was highly expressed in the stems and roots of the understory Cajanus cajan. Subsequent studies revealed that WVD2-like 4 functions as a microtubule-stabilizing protein, and Kaempferol promoted microtubule rearrangement by enhancing WVD2-like 4 transcriptional expression. WVD2-like 4 acted as a positive regulator of root growth, promoting root elongation and enhancing drought tolerance in C. cajan. This research provides novel insights into mining drought-resistant genes from understory plants and unravels the mechanism by which kaempferol regulates microtubule dynamics to enhance drought resistance in understory vegetation.
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Received: 17 May 2025
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Corresponding Authors:
**dongbiying1029@163.com; mengdongjlf@163.com
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| About author:: * These authors contributed equally to this work |
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