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| Genome-wide Linkage Analysis of Root System Architecture-related Traits in Common Wheat (Triticum aestivum) |
| TIAN Yuan-Yuan1, WANG Ya-Mei2, CHEN Lan-Jun3, JIN Yi-Rong4, LIU Peng4, MENG Xiang-Hai3*, LIU Jin-Dong5* |
1 College of Agronomy,Northwest A&F University (NWAFU), Yangling 712100, China;
2 School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China;
3 Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences, Hengshui 053000, China;
4 Dezhou Academy of Agriculture Sciences, Dezhou 253015, China;
5 Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing 100081, China |
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Abstract For a long time, the production of common wheat (Triticum aestivum) in the Huang-Huai and northern regions of China has been severely threatened by drought stress. Traits related to the root system architecture (RSA) of wheat are closely associated with the absorption and translocation of nutrients and water, which are crucial for high-yield and stable-yield of wheat. Thus, identifying loci for RSA traits and developing available markers are crucial for high and stable yield wheat breeding. In this study, RSA-related traits, including average root diameter (ARD), total root volume (TRV), and root dry weight (RDW), were evaluated in the 'Doumai'/'Shi4185' recombinant inbred line (RIL) population under hydroponics. In addition, both the RILs and parents were genotyped using the wheat 90K SNP array. Using whole genome linkage analysis, 2 loci related to ARD (QARD.caas-2B and QARD.caas-6A), 2 loci related to RDW (QRDW.caas-2A and QRDW.caas-5B), and 1 locus related to TRV (QTRV.caas-3A) were identified and each explaining 6.25%~7.59%, 7.21%~10.30% and 7.59% of the phenotypic variances, respectively. Among these, QARD.caas-6A, QRDW.caas-2A and QTRV.caas-3A were novel loci. The favorable allele of QRDW.caas-5B was contributed by the 'Doumai', whereas the favorable allele of QARD.caas-6A, QRDW.caas-2A and QTRV.caas-3A were originated from 'Shi4185'. Five candidate genes related to plant hormone regulation, stress tolerance, and signal transduction were identified, respectively. Additionally, one competitive allele-specific PCR (KASP) marker, Kasp_2A_RDW (QRDW.caas-2A) was developed and validated in 115 wheat accessions, this marker was significantly associated with wheat RDW. The study provides new genes and available KASP markers for optimizing wheat root RSA, improving wheat resistance to abiotic stress and high and stable yield breeding.
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Received: 11 December 2023
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Corresponding Authors:
*liujindong@caas.cn; mengxianghai5229@163.com
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