CD146与低氧相关因子在牦牛和黄牛脑组织差异分析

doi:10.3969/j.issn.1674-7968.2025.12.007

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Abstract Hypoxia at high altitude can cause irreversible damage to brain tissue. By comparing and analyzing the differential expressions of cluster of differentiation 146 (CD146) and hypoxia-related factors—hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), Beclin-1 and cysteine aspartate specific protease 3 (Caspase-3), in different brain tissues between yak (Bos grunniens) and yellow cattle (Bos taurus), this study explored their correlation with brain hypoxia adaptability of yak. Brain tissues (cerebrum, cerebellum, corpora quadrigemina and medulla) of adult yaks and cattle were collected for H&E, PAS, Nissl and Masson staining and immunohistochemical analysis. Based on the results of staining, various parameters were measured, including the proportion of pyramidal cells, Purkinje cell area, the distribution of glycogen accumulation, neuronal distribution and nerve fiber contents. The results showed that the differences in brain tissue between yaks and yellow cattle were mainly concentrated in the cerebellum and medulla, with all parameters in the cerebellum being significantly higher than those in the medulla (P<0.05). For interspecific differences, the average optical density (AOD) in PAS staining and the positive rate in Nissl staining were significantly higher in yak brain tissue than that in yellow cattle (P<0.05), and the proportion of nerve fibers in Masson staining was significantly higher in the yak cerebrum and cerebellum than that in yellow cattle (P<0.05), but significantly lower in the yak medulla and corpora quadrigemina (P<0.05).Immunohistochemical results showed that the differential expressions of CD146 and hypoxia-related factors were mainly in cerebellum and medulla. For inter-regional differences, the expressions of CD146 and hypoxia-related factors in the yak cerebellum were significantly higher than those in the medulla (P<0.05), whereas in yellow cattle, their expressions in the cerebellum were lower than those in the medulla. For interspecific differences, the expressions of CD146, HIF-1α, and VEGF in the yak cerebellum were higher than those in yellow cattle, while the expression trend of Beclin-1 was the opposite, the expressions of CD146 and hypoxia-related factors in the yak medulla were significantly lower than those in yellow cattle (P<0.05). Combined with the biological functions of CD146 and hypoxia-related factors, it was speculated that CD146 might play a role in the adaptation of yak brain to hypoxic environment. This study provides basic data for further exploring the specificity of hypoxia adaptation of yak brain tissue and the regulation mechanism of CD146 and hypoxia-related factors under hypoxia.

Key words： Yak Brain Cluster of differentiation 146 (CD146) Hypoxia inducible factor-1α(HIF-1α) Vascular endothelial growth factor (VEGF)

Received: 16 April 2025

ZTFLH:	S823.8+1
	S823.8+5

Corresponding Authors: * Corresponding author, 186152592@xbmu.edu.cn

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	WANG Jun
	ZHANG Fan
	XU Ming-Wei
	HU Jia-Qi
	MA Wei-Qian
	MA Si-Heng
	ZHONG Yan
	CHEN Wei-Ji
	TAN Xiao
	LAN Wen-Lin
	YANG Kun

Cite this article:

WANG Jun,ZHANG Fan,XU Ming-Wei, et al. Differential Analysis of CD146 and Hypoxia-related Factors in Yak (Bos grunniens) and Cattle (Bos taurus) Brain Tissue[J]. 农业生物技术学报, 2025, 33(12): 2626-2638.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.12.007 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I12/2626

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