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| Identification and Expression Analysis of Respiratory Burst Oxidase Homolog (RBOH) Gene Family in Blueberry (Vaccinium spp.) |
| WANG Jian-Liang1, LI Zheng-Yang1, ZHOU Yang-Yan2, JI Xing-Long1, ZHAO Qiang1,* |
1 College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China;
2 Shandong Dafengyuan Agricultural Co., Ltd., Rizhao 262305, China |
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Abstract The respiratory burst oxidase homolog (RBOH) gene family plays a crucial role in plant development and stress responses, but its members and functions in blueberry (Vaccinium spp.) remain unclear. This study aimed to identify and analyze the expression patterns of VcRBOH gene family, providing insights into its stress resistance mechanisms and molecular breeding. Based on whole-genome data of blueberry, VcRBOH gene family was identified using bioinformatics methods. The physicochemical properties of encoded proteins, gene structures, phylogenetic relationships, cis-acting elements in the promoters, and expression patterns were systematically analyzed. A total of 16 VcRBOH genes were identified on 13 chromosomes in blueberry, containing conserved NADPH (nicotinamide adenine dinucleotide phosphate) oxidase domains. Phylogenetic tree analysis classified these proteins into 3 subgroups. The results of collinearity analysis indicated that the VcRBOH gene was closely related to Arabidopsis thaliana, and there are 18 pairs of collinearity relationships. Promoter analysis demonstrated that the VcRBOH gene family promoters harbor abundant hormone-, stress-, growth and development-related regulatory elements. The transcriptome data and qRT-PCR results indicated tissue-specific expression patterns of VcRBOH genes among different cultivars. The qRT-PCR results showed that VcRBOHA, VcRBOHB, VcRBOHC, VcRBOHD, VcRBOHJ, VcRBOHK and VcRBOHL were expressed across various organs and were induced by pathogenic stresses such as anthracnose (Colletotrichum spp.) and gray mold (Botrytis cinerea). This study lays a theoretical foundation for further functional studies of disease resistance genes and resistance breeding in blueberry.
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Received: 22 April 2025
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Corresponding Authors:
* Corresponding author, zhaoqiang000666@163.com
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[1] 刘秋圆, 贺浩华, 胡丽芳. 2013. 植物Rboh基因功能及其活性调节机制的研究进展[J]. 生物技术通报, (11): 8-13.
(Liu Q Y, He H H, Hu L F. 2013. Research progress in plant Rboh# genes function and activity regulation mechanism[J]. Biotechnology Bulletin, (11): 8-13.)
[2] 潘祺, 杨立芳, 高英, 等. 2024. 蓝莓醛脱氢酶超家族成员的鉴定及表达分析[J]. 2024. 分子植物育种, 1-18.
(Pan Q, Yang L F, Gao Y, et al.2024. Identification and expression analysis of aldehyde dehydrogenase superfamily in blueberry[J]. Molecular Plant Breeding, 1-18.)
[3] 秦秀娟, 祁静静, 窦万福, 等. 2020. 柑橘Rboh家族鉴定及其对激素和柑橘溃疡病的响应[J]. 中国农业科学, 53(20): 4189-4203.
(Qin X J, Qi J J, Dou W F, e t al.2020. Identification of Rboh family and the response to hormone and citrus bacterial canker in Citrus[J]. Scientia Agricultura Sinica, 53(20): 4189-4203.)
[4] 孙旭东, 胡向阳, 杨永平. 2015. 拟南芥Rboh基因家族成员的分子和功能学比较[J].植物分类与资源学报, 37(04): 463-471.
(Sun X D, Hu X Y, Yang Y P.2015. Molecular and functional comparisons of reactive burst oxygen species gene family in Arabidopsis[J]. Plant Diversity, 37(04): 463-471.)
[5] 吴林, 胡晶, 唐雪东, 等. 2025. 我国蓝莓产业的现状与思考[J]. 中国果树, (02): 1-5.
(Wu L, Hu J, Tang X D et al. 2025. Current situation and thinking of the blueberry industry in China[J]. China Fruits, (02): 1-5.)
[6] 郑佳兴, 王艳, 王伟坤, 等. 2025. 蓝莓转录因子VcICE全基因组鉴定及其在花果发育进程的表达分析[J]. 浙江师范大学学报(自然科学版), 48(01): 51-59.
(Zheng J X, Wang Y, Wang W K, et al.2025. Genome-wide identification and relative expression analysis of transcription factor VcICE gene family in highbush blueberry during flower and fruit development[J]. Journal of Zhejiang Normal University (Natural Sciences), 48(01): 51-59.
[7] Ahammed G J, Li Z, Chen J, et al.2024. Reactive oxygen species signaling in melatonin-mediated plant stress response[J]. Plant Physiology and Biochemistry, 207: 108398.
[8] Chen C, Wu Y, Li J, et al.2023a. TBtools-II: A "one for all, all for one" bioinformatics platform for biological big-data mining[J]. Molecular Plant, 16(11): 1733-1742.
[9] Chen H, Lin Q J, Li Z, et al.2023b. Calcineurin B-like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice[J]. Molecular Plant Pathology, 24(3): 221-231.
[10] Cheng C, Xu X, Gao M, et al.2013. Genome-wide analysis of respiratory burst oxidase homologs in grape (Vitis vinifera L.)[J]. International Journal of Molecular Sciences, 14(12): 24169-24186.
[11] Colle M, Leisner C P, Wai C M, et al.2019. Haplotype-phased genome and evolution of phytonutrient pathways of tetraploid blueberry[J]. Gigascience, 8(3): giz012.
[12] Huang H L, Ullah F, Zhou D X, et al.2019. Mechanisms of ROS regulation of plant development and stress responses[J]. Frontiers in Plant Science, 10: 800.
[13] Jiang Q, Zhou X, Tang J, et al.2025. Genome-wide identification and expression profile analysis of the NADPH oxidase gene family in Avena sativa L.[J]. International Journal of Molecular Sciences, 26(6): 2576.
[14] Li X H, Zhang H J, Tian L M, et al.2015. Tomato SlRbohB, a member of the NADPH oxidase family, is required for disease resistance against Botrytis cinerea and tolerance to drought stress[J]. Frontiers in Plant Science, 6: 463.
[15] Liu J, Jiang X, Yang L, et al.2025. Characterization of the SWEET gene family in blueberry (Vaccinium corymbosum L.) and the role of VcSWEET6 related to sugar accumulation in fruit development[J]. International Journal of Molecular Sciences, 26(3): 1055.
[16] Liu M, Zhang Y, Pan T, et al.2023. Genome-wide analysis of respiratory burst oxidase homolog gene family in pea (Pisum sativum L.)[J]. Frontiers in Plant Science, 14: 1321952.
[17] Mittler R, Zandalinas SI, Fichman Y, et al.2022. Reactive oxygen species signalling in plant stress responses[J]. Nature Reviews Molecular Cell Biology, 23(10): 663-679.
[18] Morales J, Kadota Y, Zipfel C, et al.2016. The Arabidopsis NADPH oxidases RbohD and RbohF display differential expression patterns and contributions during plant immunity[J]. Journal of Experimental Botany, 67(6): 1663-1676.
[19] Neugebauer K A, Mattupalli C, Hu M, et al.2024. Managing fruit rot diseases of Vaccinium corymbosum[J]. Frontiers in Plant Science, 15: 1428769.
[20] Sagi M, Fluhr R.2006. Production of reactive oxygen species by plant NADPH oxidases[J]. Plant Physiology, 141(2): 336-340.
[21] Sharma Y, Dixit S, Singh K, et al.2024. Decoding the features and potential roles of respiratory burst oxidase homologs in bread wheat[J]. Current Plant Biology, 37: 100315.
[22] Song Z, Chen C, Duan H, et al.2025. Identification of VcRBOH genes in blueberry and functional characterization of VcRBOHF in plant defense[J]. BMC Genomics, 26(1): 153.
[23] Suzuki N, Miller G, Morales J, et al.2011. Respiratory burst oxidases: The engines of ROS signaling[J]. Current Opinion in Plant Biology, 14(6): 691-699.
[24] Wang G F, Li W Q, Li W Y, et al.2013. Characterization of rice NADPH oxidase genes and their expression under various environmental conditions[J]. International Journal of Molecular Sciences, 14(5): 9440-9458.
[25] Wang R, Li J, Liang Y.2024a. Role of ROS signaling in the plant defense against vascular pathogens[J]. Current Opinion in Plant Biology, 81: 102617.
[26] Wang W, Chen D, Liu D, et al.2020. Comprehensive analysis of the Gossypium hirsutum L. respiratory burst oxidase homolog (Ghrboh) gene family[J]. BMC Genomics, 21(1): 1-19.
[27] Wang Y, Liu Z, Li L, et al.2024b. The characteristics and expression analysis of the tomato SlRBOH gene family under exogenous phytohormone treatments and abiotic stresses[J]. International Journal of Molecular Sciences, 25(11): 5780.
[28] Wu B, Qi F, Liang Y.2023a. Fuels for ROS signaling in plant immunity[J]. Trends in Plant Science, 28(10): 1124-1131.
[29] Wu F, Zhao M, Zhang Y, et al.2023b. Systematic analysis of the Rboh gene family in seven gramineous plants and its roles in response to arbuscular mycorrhizal fungi in maize[J]. BMC Plant Biology, 23(1): 603.
[30] Zhang H, Liu X, Tang C, et al.2024. PbRbohH/J mediates ROS generation to regulate the growth of pollen tube in pear[J]. Plant Physiology and Biochemistry, 207: 108342.
[31] Zhang J, Xie Y, Ali B, et al.2021. Genome-wide identification, classification, evolutionary expansion and expression of Rboh family genes in pepper (Capsicum annuum L.)[J]. Tropical Plant Biology, 14(3): 251-266.
[32] Zhang Y, Li Y, He Y, et al.2018. Identification of NADPH oxidase family members associated with cold stress in strawberry[J]. FEBS Open Bio, 8(4): 593-605.
[33] Zhao Z, Sun A, Shan W, et al.2024. OsRbohI is the indispensable NADPH oxidase for molecular-patterns-induced reactive oxygen species production in rice[J]. Plant Communications, 5(12): 101129.
[34] Zhu Y, Su H, Liu XX, et al.2024. Identification of NADPH oxidase genes crucial for rice multiple disease resistance and yield traits[J]. Rice, 17(1): 1. |
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