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| Genetic Diversity and Structure Analysis of Seven F1 Hybrid Population of Procambarus clarkii |
| SHEN Heng-Rui1, ZHANG Ai-Fang2, WANG Qi-Shuai1, ZHOU Zhi-Yong2, LIU Yun-Long1, XU Xian-Dong2, WANG Jing-Yu2, ZHONG Ke-Er2, LI Yan-He1, HUANG Jiang-Feng2,* |
1 College of Fisheries /Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair, Huazhong Agricultural University, Wuhan 430070, China;
2 Fisheries Research Institute of Jiangxi Province, Nanchang 330039, China |
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Abstract The red swamp crayfish (Procambarus clarkii) predominate the freshwater crayfish farming field in China. To investigate the genetic diversity of the first filial generation offspring (F1) obtained from hybridization between close-distance breeding populations, the genetic diversity of 7 F1 populations derived from incomplete diallel crosses involving 4 close-distance breeding populations of P. clarkii from Jinxian (JX), Yongxiu (YX), Poyang (PY), and Henghu (HH), were analyzed using 12 pairs of microsatellite markers. The results showed that the average number of alleles (Na) for the 7 F1 populations ranged from 4.167 to 5.500, and the average effective number of alleles (Ne) ranged from 2.831 to 3.237. The average values of the Shannon's information index (I), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphic information content (PIC) ranged from 1.131 to 1.264, 0.507 to 0.607, 0.614 to 0.651, and 0.553 to 0.594, respectively, indicating a high level of genetic diversity. Genetic differentiation analysis showed that the genetic differentiation coefficient (Fst) among the F1 populations ranged from 0.004 to 0.009, the number of migrants per generation (Nm) ranged from 29.037 to 70.305, the Nei's genetic distance among the F1 populations ranged from 0.011 to 0.030, and the genetic similarity coefficient ranged from 0.971 to 0.989, indicating a low level of differentiation. The UPGMA (unweighted pair-group method with arithmetic means) clustering diagram constructed based on Nei's standard genetic distance showed that the 7 F1 populations were divided into 2 groups, with 5 F1 populations clustered into 1 cluster, and the JX♀×YX♂ group clustered with the JX♀×PY♂ group firstly, and then with the YX♀×HH♂ group, the PY♀×HH♂ group, and YX♀×JX♂ in turn, with the PY♀×JX♂ population and the HH♀×YX♂ population forming another cluster. Genetic structure analysis indicated that the optimal number of genetic clusters was K=5, and the 7 hybrid F1 populations exhibited similar and complex genetic backgrounds, with homogenization observed among them. This study provides valuable insights for diallel hybrid breeding and the utilization of hybrid offspring advantages in close-distance breeding populations of P. clarkii. and lays the foundation for the future development and utilization of high-quality germplasm resources of P. clarkii from the Poyang Lake water system.
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Received: 05 December 2024
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Corresponding Authors:
*hjf1992006@163.com
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