大黄鱼<em>atic</em>基因的分子特性及其表达量与肌苷酸含量的相关性分析

doi:10.3969/j.issn.1674-7968.2025.07.013

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Abstract The declination of flesh quality has become a major concern in the cultivation of large yellow croaker (Larimichthys crocea).For investigating the critical roles of aminoimidazole carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase (ATIC) in inosine monophosphate (IMP) formation, the relevant CDS of Lcatic was cloned and characterized. The CDS of Lcatic was 1 776 bp and encoded a polypeptide of 591 amino acids, for which the molecular weight was estimated to be 64.08 kD, with 1 N-glycosylation site and 56 phosphorylation sites. The deduced protein, LcATIC, possesses methylglyoxal synthetase (MGS) and aminoimidazole carboxamide ribonucleotide formyltransferase (AICARFT))/IMP cyclohydrolase (IMPCHase) structural domains. The genomic DNA sequence of Lcatic is subdivided into 15 exons and 14 introns, exhibiting a genome organization similar to that of atic in other teleost fish. Phylogenetic analysis of the amino acid sequence revealed that LcATIC aligned most with the Collichthys lucidus ATIC, sharing the similarity of 99.5%, they gathered in one branch and then joined the teleost branch. Lcatic mRNA was found to be expressed at a high level in the liver tissue, expressed at intermediate levels in gill, brain, intestine, and muscle tissue, and expressed at a low level in the stomach tissue. With the increase of body weight, Lcatic expression in muscle tissues of L. crocea showed a gradually increasing trend, which was consistent with the trend of gradually increasing IMP content in these muscle tissues. The Spearman correlation analysis further showed that the Lcatic mRNA expression was positively correlated with IMP content in the muscles of different-sized L. crocea (P<0.05, rs=0.931). These results suggest the critical role of Lcatic in IMP synthesis and provide basis reference for the study of the regulation mechanism of Lcatic on flesh quality of large yellow croaker.

Key words： Larimichthys crocea Inosine monophosphate (IMP) Aminoimidazole carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase (ATIC) Molecular characterization

Received: 02 September 2024

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Corresponding Authors: ^*shixiaoli209@163.com

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	HE Liang-Yin
	ZHANG Ya-Ting
	YOU Yu-Xin
	ZENG Zhi-Jun
	SUN Zhao-Han
	SHI Xiao-Li

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HE Liang-Yin,ZHANG Ya-Ting,YOU Yu-Xin, et al. Molecular Characterizations of atic Gene and the Correlation Analysis Between Its Expression Levels and Inosine Monophosphate Content in Large Yellow Croaker (Larimichthys crocea)[J]. 农业生物技术学报, 2025, 33(7): 1568-1579.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.07.013 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I7/1568

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