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| Analysis of Copy Number Variation of PPP3CA Gene and Its Association with Growth Traits in Goats (Capra hircus) |
| BAI Yang-Yang1, DU Shu-Zeng3, LI Xin1, ZHU Hai-Jing4, QU Lei4, GUO Zhen-Gang5, YANG Qing1,2,*, LAN Xian-Yong1,* |
1 College of Animal Science and Technology, Northwest A&F University, Yangling 712000, China;
2 Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, China;
3 Nanyang Agricultural Vocational College, Nanyang 473000, China;
4 Shaanxi Cashmere Goat Engineering Research Center, Yulin University, Yulin 719000, China;
5 Bijie Institute of Animal Science and Veterinary Science, Bijie 551700, China |
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Abstract Protein phosphatase 3 catalytic subunit alpha (PPP3CA) plays an important role in animal growth and development. This study aims to explore PPP3CA gene expression in different tissues and the genetic effects of copy number variation on the growth traits of different breeds of goats (Capra hircus). First, a total of 11 Shaanbei white cashmere goats were chosen to identify the expression levels of PPP3CA mRNA in the heart, liver, spleen, lung, kidney, muscle, brain, ovary, testis tissues. Then, copy number variation (CNV) of PPP3CA gene was detected in 306 Shaanbei white cashmere goats and 110 Guizhou Heima goats by qPCR. The associations between PPP3CA gene CNV loci and growth traits in goats were analyzed using ANOVA. In addition, the linkage disequilibrium block in these regions and the potential transcription factors that could bind to the 3 CNVs were analyzed by using bioinformatics. The results showed that PPP3CA mRNA was highly expressed in the lung, followed by the spleen, fallopian tube, testis, heart, liver, kidney, muscle and brain. Three CNVs were detected in PPP3CA gene. In the Shaanbei white cashmere goats population, CNV1 and CNV2 significantly correlated with goat chest circumference (P<0.05); CNV3 was significantly correlated with goat chest width, chest depth and chest circumference (P<0.05). In the Guizhou Heima goat population, there was no significant relationship between 3 CNVs and goat growth traits. The linkage disequilibrium (LD) haplotype block analysis showed that there was no LD block for PPP3CA gene, but these CNVs might bind to SPT23, SIP4, and YOX1 transcription factors. In summary, these findings indicated that the CNV1, CNV2, and CNV3 loci of the PPP3CA gene could be used as molecular markers for the selection of growth traits in Shaanbei white cashmere goats.
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Received: 07 July 2023
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