长期有机种植模式下日光温室蔬菜栽培土壤真菌多样性研究

doi:10.3969/j.issn.1674-7968.2024.03.014

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Abstract Organic cultivation can improve soil fertility and biodiversity by protecting soil organic matter. Greenhouse cultivation can artificially control the growing environment of plants and protect crops from outdoor disturbances such as pests and diseases, extreme weather conditions and pesticide drift. In this study, two different land use methods (solar greenhouse and open field vegetable cultivation)(0~15) cm top soil and (15~30) cm deep soil were used as research objects, and Illumina sequencing was utilized to analyze the variations in soil microbial community structure. Key soil environmental factors affecting fungal community structure were identified through dbRDA analysis. Meantime, FUNGuild was used to analyze the response of soil fungal function to solar greenhouse cultivation.The results showed that organic cultivation in greenhouse significantly reduced (P<0.05) the richness and diversity of topsoil fungal communities. The dominant fungi of different land-use mode were Ascomycota, Glomeromycota and Basidiomycota. Although there was no change in the species of dominant fungi in the soil due to different land-use modes, their relative abundance was significantly influenced. Solar greenhouse cultivation increased the number and abundance of beneficial fungi such as Chaetomium, Mortierella, Orbilia, Acaurospora, and inhibited the growth of plant pathogenic fungus Fusarium in the surface soil. LEfSe analysis showed that 22 and 21 different species were detected in greenhouse and open soil, respectively. The Mortierellomycota, Rozellomycota, and Chaetomium, Mortierella, and Humicola fungal genera in greenhouse soil showed significant differences in microbial communities. Fusarium, Stachybotry, and Cephaliophora played a dominant role in the open field cultivation. dbRDA analysis showed that total potassium (TK), soil organic matter (SOM), pH, total nitrogen (TN) and total phosphorus (TP) were the environmental factors that significantly affected the soil fungal community in solar greenhouse. FunGuild function analysis showed that the most advantageous nutrient type in greenhouse soil was Saprotroph, while in open feiled was Pathotroph-Saprotroph.Thus, greenhouse organic vegetable cultivation could significantly affect the fungal community structure, improve the abundance of beneficial fungi in soil and reduce the content of pathogenic fungi, and adaptation of changes in fungal ecological functions to environmental influences caused by different land-use patterns. This study revealed the effect of vegetable cultivation in solar greenhouse on the structure and function of soil fungal community under long-term organic cultivation mode, and will provide theoretical basis for the composition of soil fungal community and long-term organic cultivation under greenhouse conditions.

Key words： Organic planting Solar greenhouse High-throughput sequencing Fungal community diversity

Received: 04 April 2023

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Corresponding Authors: ** gsg1668@163.com

About author:: * These authors contributed equally to this work

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	ZENG Guang-Juan
	WANG Han
	ZHAO Mei-Wei
	PENG Hong-Li
	FENG Yang
	GENG Shi-Gang

Cite this article:

ZENG Guang-Juan,WANG Han,ZHAO Mei-Wei, et al. Study on the Diversity of Soil Fungi in Vegetable Cultivation in Solar Greenhouse Under Long-term Organic Planting[J]. 农业生物技术学报, 2024, 32(3): 641-654.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2024.03.014 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2024/V32/I3/641

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