Abstract：Forty-nine isolates of Trichoderma were collected and isolated from spawn and fruiting body of edible fungi. They were identified based on morphological taxonomy proposed by Gams & bissett (1998) and ITS analysis. The results showed that Trichoderma species associated with cultivated edible fungi from Fujian and Zhejiang of China were dominated by T. harzianum and T. longibrachiatum , but T. atroviride and T. asperellum were rare. Species of Trichoderma varied with collection locality and mushroom species concerned. Trichoderma isolated from spawn of Lentinula edodes was dominated by T. harzianum in Qingyuan country of Zhejiang province.Trichoderma isolated from spawn of Pleurotus eryngii was dominated by T. asperellum in Guangzhou, while that isolated from spawn of cultivated lentinula edodes was dominated by T. atroviride in Pucheng of Fujian province. The result of morphological identification and that of ITS analysis was approximately coincident
