Antimicrobial Stability of Bacillus subtilis Strain Rbac1 and Its Biocontrol Effect on Plant Anthracnose
HU Qian1,*, JIANG Hao2,*, LIU Yang3, WANG Yi1, HE Lu-Qian1, KONG Li-Ya1, WANG Zhan-Qi1, ZHANG Li-Qin1, MIN Li-Jing1, TANG Zhao-Yang1,**
1 College of Life Sciences/Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Huzhou Normal University, Huzhou 313000, China; 2 National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, China; 3 Qujiang District Bureau of Agriculture and Rural Affairs of Quzhou City, Quzhou 324000, China
Abstract：Anthracnose poses a severe threat to the green and sustainable development of the agricultural industry. To explore novel biological control technologies, a Bacillus subtilis strain Rbac1 was isolated and purified in this study. The control efficacy of strain Rbac1 against various important diseases, especially anthracnose, was investigated. Results showed that Rbac1 strain significantly inhibited multiple pathogens, including Colletotrichum camelliae, Co. fructicola, Fusarium solani, Aspergillus flavus, and Phytophthora sojae. The conidial germination test indicated that the fermentation filtrate of strain Rbac1 remarkably suppressed the conidial germination of Co. camelliae and Co. fructicola. The antibacterial stability test revealed that the fermentation broth of this strain exhibited favorable thermal stability. There was no significant difference in inhibition rates at pH 3~9 and no obvious change following a 60 min ultraviolet irradiation treatment. The detached leaf biocontrol test demonstrated that the fermentation filtrate of strain Rbac1 effectively controlled anthracnose in 'Baiye 1' and 'Huang jinya' tea(Camellia sinensis) varieties, with control efficiencies of 58.03% and 70.99% respectively. In conclusion, B. subtilis Rbac1 showed great potential for biological control of tea anthracnose. This study provides theoretical basis and strain resources for the development of novel biological control agents.
胡骞, 姜浩, 刘洋, 王怡, 何鲁钱, 孔丽娅, 王占旗, 张立钦, 闵莉静, 唐朝阳. 枯草芽胞杆菌Rbac1的抑菌稳定性及其对植物炭疽病的生防效果[J]. 农业生物技术学报, 2026, 34(8): 1766-1776.
HU Qian, JIANG Hao, LIU Yang, WANG Yi, HE Lu-Qian, KONG Li-Ya, WANG Zhan-Qi, ZHANG Li-Qin, MIN Li-Jing, TANG Zhao-Yang. Antimicrobial Stability of Bacillus subtilis Strain Rbac1 and Its Biocontrol Effect on Plant Anthracnose. 农业生物技术学报, 2026, 34(8): 1766-1776.
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