Verification of the Secretory Property of AFABP in Chickens (Gallus gallus) and Its Regulatory Effect on Preadipocyte Proliferation
GAO Fan, GUO Ya-Qi, XU Hai-Dong, MOU Fang, WANG Ning*
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province/College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Abstract：Adipocyte fatty acid-binding protein (AFABP), a member of the fatty acid-binding protein family, is predominantly expressed in adipocytes, macrophages, and endothelial cells. While traditionally regarded as an intracellular fatty acid transporter, mammalian AFABP has been identified as a secretory adipokine that regulates systemic metabolism via the circulatory system. However, it remains unclear whether chicken (Gallus gallus) AFABP exhibits similar secretory property and possesses extracellular functions. In this study, Western blot analysis was used to verify the secretory capacity of chicken AFABP; CCK8 and RT-qPCR assays were used to examine its effect on chicken preadipocyte proliferation. Western blot analysis showed that chicken AFABP protein was present in the culture supernatants of the transfected cells, indicating that AFABP possessed secretory property. CCK8 and RT-qPCR assays revealed that the secreted chicken AFABP promoted preadipocyte proliferation. This study provides a foundation for further investigation into the function and mechanism of chicken AFABP.
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