Abstract：In recent years, the application of traditional Chinese medicine has become more and more popular, and the market demand is increasing, but the mixed phenomenon of counterfeits is serious. The development of identification kits based on DNA detection technology is of significant practical significance for this medicinal material. This study was based on the phylogenetic tree constructed in early stage of the experiment on A. edulis and its fakes, and specific PCR primers for A. edulis were designed on the basis of internal transcribed spacers (ITS) sequence analysis to optimize the use of specific detection reagents and reaction conditions. According to the evaluation criteria of in vitro diagnostic kits, a test kit was developed for A. edulis, and the test kit was evaluated for specificity, detection limit, repeatability, shelf life and stability. According to the test results, the kit had good specificity, the detection limit was 0.03 μg/μL, and excellent stability. The specific identification kit of authentic Chinese medicinal material A. edulis was accurate, fast and effective for the identification of A. edulis, and has broad application prospects.
孙传伯, 何燕飞, 张晶, 王玉威, 赵群. 道地中药材光慈菇检测试剂盒的研制和评价[J]. 农业生物技术学报, 2022, 30(6): 1202-1209.
SUN Chuan-Bo, HE Yan-Fei, ZHANG Jing, WANG Yu-Wei, ZHAO Qun. Development and Evaluation of the Detection Kit for Authentic Chinese Medicinal Materials Bulb of Edible Tulip. 农业生物技术学报, 2022, 30(6): 1202-1209.
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