Polymorphisms of FGF21 Gene and Their Association Analysis with Growth and Carcass Traits in Ovine (Ovis arise) with Different Genders
AN Qing-Ming1,2, WU Zhen-Yang2, MENG Jin-Zhu1, ZHAO Yuan-Yuan1, WANG Xing1, LUO Yu-Zhu2,*
1 College of Agriculture and Forestry Engineering and Planning, Tongren University, Tongren 554300, China; 2 Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Fibroblast growth factor 21 (FGF21) is an important endocrine regulatory protein factor, which plays an important role in ontogenesis and metabolism. In order to analyze the effect of polymorphisms of ovine FGF21 gene on growth traits and carcass traits in lambs (Ovis arise), to filter variation of FGF21 gene. Blood samples of 5 different commodity sheep were collected, including 170 sheep were tested for polymorphism, 756 Romney lambs were analyzed for correlation which relevant production data were collected. DNA were extracted and polymorphisms in Exon-1 and Exon-3 regions of FGF21 gene were detected by PCR-single strand conformation polymorphism (PCR-SSCP), and the relationship between allele of Exon-3 regions and production traits in lambs was investigated using generalized linear models (GLMs). In total, 6 SNPs were detected in Exon-1 and Exon-3 regions of ovine FGF21 gene, the nucleotide substitution c.554 C/T in Exon-3 resulted in amino acid change (p.Pro185Leu). The association analysis results showed that the allele in Exon-3 region of ovine FGF21 gene had different effects on growth traits of male and female lambs. In male lambs, the presence of A2 was associated with decreased tailing weight and weaning weight (P<0.05), and in female lambs the presence of B2 was associated with decreased weaning weight and pre-weaning growth rate (P<0.05). No associations were detected between the other allele and growth traits in population. Meanwhile, the results showed that the presence of A2 was associated with decreased hot-carcass weight (H-W), the presence of B2 was associated with increased H-W, should yield and total yield (P<0.05). No associations were detected between the other allele and carcass traits in male lambs. Ovine FGF21 gene had abundant polymorphisms. The allele of Exon-3 of ovine FGF21 gene might have different effects on growth traits of male and female lambs, and also might affect some carcass traits, which could improve the growth traits and carcass traits of lambs, and provide basis molecular genetic data for improving important economic traits of sheep, FGF21 gene could be an effective molecular marker for marker-assisted breeding of sheep, these results could provide scientific basis for genetic improvement of sheep.
安清明, 吴震洋, 孟金柱, 赵园园, 王星, 罗玉柱. FGF21基因多态性与不同性别绵羊生长性状和胴体性状的关联分析[J]. 农业生物技术学报, 2021, 29(2): 298-306.
AN Qing-Ming, WU Zhen-Yang, MENG Jin-Zhu, ZHAO Yuan-Yuan, WANG Xing, LUO Yu-Zhu. Polymorphisms of FGF21 Gene and Their Association Analysis with Growth and Carcass Traits in Ovine (Ovis arise) with Different Genders. 农业生物技术学报, 2021, 29(2): 298-306.
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