哺乳动物多能干细胞向生殖细胞分化的研究进展

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摘要多能干细胞具有自我更新能力及多向分化潜能。生殖细胞是遗传信息的携带者。将多能干细胞定向分化为生殖细胞,不仅为研究生殖发育及器官发生发育奠定坚实的基础,也可为人类(Homo sapiens)不孕不育的治疗及优良动物品种的保存提供新思路和方法。目前,小鼠(Mus musculus)多能干细胞向生殖细胞诱导分化的研究最为成熟,可在体外获得功能性的生殖细胞; 人及猪、牛、羊等家畜动物多能干细胞向生殖细胞诱导分化体系尚不成熟,仍需进一步优化。本文主要对小鼠、人及家畜动物多能干细胞向生殖细胞诱导分化的方法、诱导体系及目前所取得的进展进行综述,并对诱导分化中存在的问题及研究策略进行了展望,为实现猪、牛、羊等家畜大动物多能干细胞向生殖细胞诱导分化提供理论依据。

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	相金柱
	王寒凝
	李雪玲

关键词 ：小鼠, 人, 多能干细胞, 生殖细胞, 定向分化

Abstract：Pluripotent stem cells have the ability of self-renewing and multiple differentiation potential. Germ cells are the carriers of genetic information. Direct differentiation of germ cells from pluripotent stem cells not only lays the foundation of studying reproductive development and organogenesis,but also provides new ideas and methods of treating human (Homo sapiens) infertility and conserving excellent animal breeds. At present,the study of induced differentiation of mouse (Mus musculus) pluripotent stem cells into germ cells is the most mature,and functional germ cells can be obtained in vitro; the system of induced differentiation of human,pig,cattle,sheep and other livestock pluripotent stem cells into germ cells is not mature,which needs further optimization. In this paper,the methods and systems of inducing differentiation of mouse,human and livestock pluripotent stem cells into germ cells are reviewed,and the problems and research strategies in inducing differentiation are prospected. This review provides a theoretical basis for the differentiation of domestic animal pluripotent stem cells into germ cells.

Key words： Mouse Human Pluripotent stem cells Germ cells Directional differentiation

收稿日期: 2020-01-15

ZTFLH:

S814.1

基金资助:内蒙古自然科学基金(2020BS03003,2020BS03022);内蒙古自然基金重大项目(2020ZD10);内蒙古科技重大专项(2020ZD0007);内蒙古科技厅重大专项(zdzx2018065);国家自然基金(31460309)

通讯作者: **lixueling@imu.edu.cn

引用本文:

相金柱, 王寒凝, 李雪玲. 哺乳动物多能干细胞向生殖细胞分化的研究进展[J]. 农业生物技术学报, 2020, 28(11): 2061-2068.
XIANG Jin-Zhu, WANG Han-Ning, LI Xue-Ling. Research Progress in Differentiation of Germ Cells from Mammalian Pluripotent Stem Cells. 农业生物技术学报, 2020, 28(11): 2061-2068.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I11/2061

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