Study on PRLR Gene Expression and Tissue Distribution in Pituitary and Ovary of Ganjia Tibetan Sheep (Ovis aries) During Estrous Cycle
ZHOU Kai-Ren1,2, HE Yu-Qin1,*, GE Wen-Bo1, ZHANG Quan-Wei1, YANG Da-Peng1, WANG Xia1, CHEN Wei-Gang1, BAO Ying-Ying1
1 College of Life Sciences and Technolog, Gansu Agricultural University, Lanzhou 730070, China; 2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Ganjia Tibetan sheep (Ovis aries) are endemic animal in Gansu, and the reproduction rate is low due to the particularity of their living environment. In order to study the expression and distribution of prolactin receptor (PRLR) in pituitary and ovarian tissues of Ganjia Tibetan sheep during estrous cycle and illuminate the regulation of gene expression in reproductive activity, 32 Ganjia Tibetan sheep were selected, and the expression and distribution of PRLR in pituitary and ovarian tissues during estrous cycle were detected by immunohistochemical staining and real-time fluorescent quantitative PCR. The results showed that PRLR and its gene expression were found in the pituitary and ovarian tissues of estrous cycle of Ganjia Tibetan sheep. In the pituitary, the gene expression of PRLR in oestrus was the highest, which was significantly higher than that in the metestrus (P<0.05); PRLR-positive cells were mainly distributed in the cytoplasm of the distal eosinophils of the pituitary gland, and PRLR had the most distribution in the proestrus and the least in the oestrus. In the ovarian, PRLR gene expression was highest in oestrus, significantly higher than that in metestrus, proestrus and diestrus (P<0.05); Positive PRLR cells were mainly distributed in follicular granule layer, and had the most distribution in proestrus and the least in estrus period, and the pre-estrus and inter-estrus period had more distribution than that in post-estrus period and estrus period (P<0.05).The distribution and gene expression of PRLR in pituitary and ovary tissues of Ganga Tibetan sheep during estrous cycle were different, suggesting that there might be differences in the regulation of PRLR on estrous cycle. The results of this study provide basic data for the regulation of reproductive performance of Tibetan sheep.
周凯仁, 何玉琴, 葛闻博, 张全伟, 杨大鹏, 王霞, 陈卫刚, 包莹莹. 甘加藏羊发情周期垂体和卵巢中PRLR基因表达与组织分布的研究[J]. 农业生物技术学报, 2019, 27(10): 1894-1900.
ZHOU Kai-Ren, HE Yu-Qin, GE Wen-Bo, ZHANG Quan-Wei, YANG Da-Peng, WANG Xia, CHEN Wei-Gang, BAO Ying-Ying. Study on PRLR Gene Expression and Tissue Distribution in Pituitary and Ovary of Ganjia Tibetan Sheep (Ovis aries) During Estrous Cycle. 农业生物技术学报, 2019, 27(10): 1894-1900.
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