大口黑鲈PACAP基因SNPs的筛选及其与生长性状的关联分析

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1415 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要垂体腺苷酸环化激酶多肽(pituitary adenylate cyclase-activating polypeptide, PACAP)是一种能够促进生长激素、促性腺激素和催乳素分泌的多效激素，属于胰高血糖素/分泌肽家族的成员。为探索PACAP基因多态性对大口黑鲈(Micropterus salmoides)生长性状的影响，本研究采用直接测序的方法在PACAP基因上检测到一个单核苷酸多态性(single nucleotide polymorphism, SNP)位点：A-2282C，基因型检测共发现3种基因型：AA、AC和CC，其基因型频率分别为0.034、0.645和0.321，A等位基因频率为0.356 3，C等位基因频率为0.643 7。随机选取同批繁殖、同塘养殖的327尾大口黑鲈用Snapshot方法进行SNPs位点检测和分型，构建最小二乘分析模型，分析基因型与生长性状的相关性。χ2检测结果显示，哈温-平衡常数为0.061 5，即该位点在所检测大口黑鲈群体中基本处于Hardy-Weinberg平衡状态, 有效等位基因数(Ne)为1.846 5，期望杂合度(Ho)和观测杂合度(He)分别为0.540 9和0.644 2。方差分析结果显示，AC基因型群体的平均体质量、体高和全长相对于AA基因型群体分别增长了30.6%、10.7%和8.3%，相对于CC基因型群体分别增长了10.5%、4.7%和2.7%；且AC基因型群体在体质量、体高和全长上显著高于AA和CC基因型群体(P＜0.05)。另外，虽然AA基因型群体的平均体质量、体高和全长相对于CC基因型群体分别增长了18.1%、5.8%和5.4%，但AA型群体和CC群体在各生长性状上没有显著差异(P＞0.05)。该位点可能是影响大口黑鲈生长性状的的主效数量性状核苷酸(quantitative trait nucleotides, QTN)或与之紧密连锁，可作为大口黑鲈选育的候选分子标记。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	刘浩
	白俊杰
	李胜杰
	马冬梅
	全迎春

关键词 ：大口黑鲈, 垂体腺苷酸环化激酶多肽(PACAP), SNP, 生长

Abstract：Pituitary adenylate cyclase-activating polypeptide (PACAP), a member of glucagon/secretes peptide family, is a pleiotropic hormone that enhances and stimulates the secretion of growth hormone, gonadotropin, prolactin and somatolactin. The single nucleotide polymorphism (SNP) in PACAP gene can affect its function or translation and transcription. Studying the association between the SNP and growth traits can provide candidate markers for marker-assisted selection. We detected the polymorphism of PACAP gene by DNA sequencing to explore the association between the polymorphism of PACAP and growth traits of largemouth bass (Micropterus salmoides). Five pairs of primers were designed to amplify the PACAP gene sequence, and a SNP A-2282C site was found in the 4th intron, and had 3 genotypes named as AA, AC and CC, respectively. The genotype and gene frequency of this SNP in 327 largemouth bass were further assayed with the method of Snapshot, the result showed that gene frequency of genotypes AA, AC and CC were 0.034, 0.645 and 0.321, respectively, and the frequency of A allele and C allele were 0.355 8 and 0.644 2. Chi-square test showed that the locus was in Hardy-Weinberg equilibrium in the largemouth bass population，the effective number(Ne) of the locus was 1.846 5, expected heterozygosity (Ho) and observed heterozygosity (He) were 0.540 9 and 0.644 2. The least squire method (LSM) was used to analyze the association of genotype with growth traits, and variance analysis indicated the mean values of AC genotype group was higher than that of AA and CC groups in body weight, body length, head length, total length, body depth and caudal peduncle length. And the body width and caudal peduncle length with AC genotype were significantly higher than that of AA (P＜0.05). The depth with AC genotype was extremely significantly higher than group with CC (P＜0.01), and those with AC genotype were significantly higher than those with AA genotype (P＜0.05). The locus can be a candidate molecular marker for largemouth bass breeding.

Key words： Largemouth bass Pituitary adenylate cyclase-activating polypeptide (PACAP) SNP Growth

收稿日期: 2015-09-10 出版日期: 2016-02-05

ZTFLH:	Q 753
	S 917

基金资助:国家科技支撑计划;国家自然科学基金;948计划重点项目“水产主导品种分子育种与基因组选择育种技术引进”

通讯作者: 白俊杰 E-mail: baijj2005@21cn.com;jjbai@163.net

引用本文:

刘浩白俊杰李胜杰马冬梅全迎春. 大口黑鲈PACAP基因SNPs的筛选及其与生长性状的关联分析[J]. , 2016, 24(3): 390-396.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2016/V24/I3/390

郝瑞杰,昝林森,王洪宝,等.2010.垂体腺苷酸环化激酶多肽基因(PACAP)G3909A突变对秦川牛部分生长性状的影响[J].农业生物技术学报,18(4):719-724.(Hao Ruijie, Zan Linsen, Wang Hongbao, et al.2010.Effect of G3909A Mutation in Pituitary Adenylate Cyclyse-activating Polypeptide Gene(PACAP) on the Partial Growth Traits in Qinchuan Cattle[J].Journal of Agricultural Biotechnology,18(4):719-724.)
李俊营,周志鹏,詹凯,等.2015.PACAP基因C1049A位点遗传多态性及其与淮南麻黄鸡产蛋性能的相关性[J].中国家禽.17(3):9-12.(Li Junying, Zhou Zhipeng, Zhan Kai, et al.2015.Genetic Polymorphism of PACAP Gene at C1049A Site and Its Association with Laying Performance of Huainan Partridge Chicken[J].China Poultry,17(3):9-12)
李胜杰, 白俊杰, 叶星, 等.2007.加州鲈肌肉生长抑制素(MSTN) cDNA 的克隆和序列分析[J]. 海洋渔业, 29(1): 13-19.(Li Shengjie, Bai Junjie, Ye X, et al.2007.Molecular cloning and sequence analysis of Myostatin in largemouth bass (Micropterussal moides) [J]. Marine Fisheries, 29(1): 13-19.)
李玺洋,白俊杰,于凌云,等.2012.草鱼醛缩酶 A 3'-UTR 突变与生长性状相关研究[J]. 淡水渔业, 42(05):13-16.(Li Xiyang, Bai Junjie, Yu Lingyu, et al.2012.Association analysis between mutation of aldolase A 3′-UTR and growth traits in Ctenopharyngodon idella[J].Freshwater Fisheries,42(05):13-16.)
刘桂兰,蒋思文,熊远著,等. 2003.IGF2 基因PCR-RFLP多态性与脂肪沉积相关性状的关联分析[J].遗传学报,30(12):1107-1112.(Liu Guilan, Jiang Siyuan, Xiong Yuanzhu, et al.2003.Association of PCR-RFLP Polymorphism of IGF2 Gene with Fat Deposit Related Traits in Pig Resource Family[J]. Journal of Genetics and Genomics,30(12):1107-1112.)
陶文静, 马龙俊, 阮瑞霞, 等.2011.建鲤GHR基因多态性及与增重相关的SNP位点的筛选[J].水生生物学报, 35(4): 622-629.(Tao Wenjing, Ma Longjun, Yuan Ruixia, et al.2011.SNP Loci Association With Weight Gain on Growth Hormone Receptor Genes in CYPRINUS Var.Jian[J].2011.Acta Hydrobiologica Sinic,35(4):622-629.)
岳双明,王之盛,周安国.2008.PACAP对动物采食量和摄食行为的影响[J].中国畜牧杂志. 44(13):52-56.(Yue Shuangming, Wang Zhisheng, Zhou Anguo, et al.2008.Effect of PACAP on animal Feed Intake and Feeding Behaviors[J].Chinese Journal of Animal Science,44(13):52-56.)
Charoensook R, Gatphayak K, Sharifi AR, et al.2012.Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle [J]. Tropical Animal Health Production, 44(4):921-928.
Green B.D., Irwin N., Flatt P.R.2006.Pituitary adenylate cyclase-activating peptide (PACAP):Assessment of dipeptidy1 peptidase IV activity and antidiabetic potential[J]. Peptides, 27(6):1349-1358.
Guiwei Zou, Yuanyuan Zhu, Hongwei Liang, et al.2015.Association of pituitary adenylate cyclase-activating polypeptide and myogenic factor 6 genes with growth traits in Nile tilapia (Oreochromis niloticus)[J]. Aquaculture International, 23(5):1217-1225.
Jiang Y, Li W S, Lin H R.2005. The prokaryotic expression and biological activity of the pituitary adenylate cyclase activating polypeptide in groupers Epinephelus coioides[J]. Acta Zoologica Sinica, 51: 1162-1166.
Jarry H, Leonhardt S, Schmidt WE, et al.1992.Contrasting effects of pituitary adenylate cyclase activating polypeptide (PACAP) on in vivo and in vitro prolactin and growth hormone release in male rats[J]. Life Sciences, 51(11):823-830.
Juszczuk-kubiak E, Sakowski T,Flisikowski K, et al.2004. Bovine mu-calpain(CAPN1)gene: new SNP within intron 14[J]. Journal of Applied Genetics, 45(4):457-460.
Klett C.P.R., Bonner T.L.1999.Identification and characterization of the rat M1 muscarinic receptor promoter[J]. Journal of Neurochemistry, 72(3):900-909.
Lugo J M, Rodriguez A, Helguera Y, et al.2008. Recombinant novel pituitary adenylate cyclase-activating polypeptide from African catfish(Clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates[J]. Journal of Endocrinology, 197: 583-597.
Li Shengjie, Han Linqiang, Bai Junjie, et al.2015.Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass[J]. Chinese Journal of Oceanology and Limnology. 33 (2):328-338.
Montero M, Yon L, Rousseau K, et al.1998.Distribution,Characterization,and Growth Hormone-releasing Activity of Pituitary Adenylate Cyclase-Activating Poly-peptide in the European Eel, Anguilla Anguilla 1[J]. Endocrinology, 139(10): 4300-4310.
Matsuda K, Nejigaki Y, Satoh M, et al.2008.Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on prolactin and somatolactin release from the goldfish pituitary in vitro[J]. Regulatory Peptides, 145(1-3):72-79.
Mukai K., Takeuchi T., Toyoshima M., et al.2006.PACAP and PHI-mediated Sustained Relaxation in Circular Muscle of Gastricfundus: Findings Obtained in PACAP Knockout Mice[J]. Regulatory Peptides, 133 (1-3):54-61.
Miyata A, Arimura A,Dahl R.R. et al.1989. Isolation of a novel 38 residue hypothalamic polypeptide which stimulates adenylate cyclase in pituitary cells. Biochemical and Biophysical Research Communications, 164(1): 567-574.
Money J E, Michael H, Patricia M. et al.1992. Pituitary Adenylate cyclase activating polypeptide (PACAP) reduces food intake inmice[J]. Peptides, 13:1133-1135.
Matsuda K, Maruyama K, Nakamachi T, et al.2006.Effects of pituitary adenylate cyclase- activating polypeptide and vasoactive Intestinal polypeptide on food Intake and locomotor activity in the goldfish, carassius auratus[J].Annals of the New York Academy Sciences, 1070(1): 417-421.
Nakata M., Kohno D., Shintani N., et al.2004.Yada T. PACAP deficient mice display reduced carbohydrate intake and PACAP activates NPY-containing neurons in the rat hypothalamic arcuate nucleus[J].Neuroscience Letters, 370(3): 252-256.
Nakata M, Kohno D, Shintani N, et al.2004.PACAP deficient mice display reduced carbohydrate intake and PACAP activates NPY-containing neurons in the rat hypothalamic arcuate nucleus [J].Neuroscience Letters, 370(3): 252-256.
Piqueras L., Tachi Y., Martinet V.2004.Peripheral PACAP inhibits gastric acid secretion through somatostatin release in mice[J].British Journal of Pharmacology, 142(1): 67-78.
Q. J. Jin, X. T. Fang, C. L. Zhang, et al.2010. A novel SNP of the GHRL gene in goat and its association with growth traits[J]. Small Ruminant Research, 90:150-152.
Tachibana T, Saito S, Tomonaga S, et al. 2003.Intracerebroventricu?lar injection of vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide inhibits feeding in chicks[J].Neuroscience Letters, 339:203-206.
Tao WJ, Boulding EG.2003.Associations between single nucleotide polymorphisms in candidate genes and growth rate in Arctic charr (Salvelinus alpinus L.)[J]. Heredity, 91:60-69.