1 Cotton Research Institute, the Shihezi Academy of Agriculture Sciences, Shihezi 832000, China; 2 Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250000, China; 3 School of Life Sciences, Henan University, Kaifeng 475001, China
Abstract：Filamentation temperature-sensitive H(FtsH), which possesses ATPase and proteolytic activities, is widely distributed in plants and plays important roles in plant growth and development, as well as in plant responses to stress. In this study, the function of FtsH family members in the growth, development, and salt stress response of Gossypium hirsutum were systematically explored, 39 GhFtsH genes were identified in the whole genome of G. hirsutum and were analyzed using bioinformatics methods. The expression profiles of GhFtsH gene family members in various tissues were constructed using RNA-seq data. qRT-PCR was employed to detect the expression patterns of 'Lumianyan 37' across distinct developmental stages under different salt concentration treatments. The results revealed that the 39 GhFtsH genes were unevenly distributed on 19 chromosomes. Phylogenetic analysis showed that 39 GhFtsH genes were divided into 7 subgroups. All GhFtsH proteins contained 2 typical conserved motifs. Synteny analysis revealed that several GhFtsH genes were collinear with Arabidopsis thaliana and rice(Oryza sativa). Promoter sequence analysis of GhFtsH identified the cis-acting elements associated with photo-response and stress responses. Most GhFtsH genes were highly expressed in leaves. Similarly, GhFtsH1, GhFtsH2, GhFtsH14, and GhFtsH15 maintained high expression levels under abiotic stress. qRT-PCR results revealed that under salt stress the expression levels of GhFtsH1, GhFtsH2 and GhFtsH14 differed with treatment concentration and developmental stage, with GhFtsH2 exhibiting the most sensitive response. This study provides an important theoretical basis for elucidating the molecular mechanisms of salt tolerance and for stress-resistance breeding in cotton.
任贺丽, 张乐, 唐秉晖, 高阳, 李玉国, 张军, 艾尼江. 陆地棉FtsH基因家族的鉴定及其在盐胁迫下的表达分析[J]. 农业生物技术学报, 2026, 34(8): 1628-1644.
REN He-Li, ZHANG Le, TANG Bing-Hui, GAO Yang, LI Yu-Guo, ZHANG Jun, Ainijiang. Identification of the FtsH Gene Family in Gossypium hirsutum and Its Expression Analysis Under Salt Stress. 农业生物技术学报, 2026, 34(8): 1628-1644.
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