Identification and Expression Analysis of the PRX Gene Family in Tobacco (Nicotiana tabacum) in Response to Ralstonia solanacearum Infection Under DADS Treatment
CHEN Jia-Li, DENG Wen-Qi, YANG Fan*
College of Tobacco Science/Guizhou Provincial Key Laboratory for Tobacco Quality Improvement and Efficiency Enhancement, Guizhou University, Guiyang 550000, China
Abstract：Class Ⅲ peroxidases (PRXs) play essential roles in plant stress responses and immune regulation. To investigate the expression patterns and functional roles of PRXs in tobacco (Nicotiana tobacum) resistance against bacterial wilt induced by diallyl disulfide (DADS), this study conducted a systematic identification and analysis of the tobacco PRX gene family based on previous transcriptome data (NCBI accession number: PRJNA1470963). A total of 19 NtPRX genes were identified, unevenly distributed on 12 chromosomes. All encoded proteins contained typical secretory peroxidase domains, and their promoter regions were enriched with hormone-responsive, light-responsive, and stress-related cis-acting elements. Phylogenetic, conserved motif, and collinearity analyses indicated that the NtPRX gene family was evolutionarily conserved yet exhibited functional diversification. Furthermore, combining transcriptome data from roots and leaves of tobacco pretreated with DADS and subsequently inoculated with Ralstonia solanacearum, and validating with qRT-PCR. The result showed that DADS pretreatment could induce high-level expression of NtPRX4, NtPRX9, NtPRX11, NtPRX13, and NtPRX19 in both roots and leaves, and significantly enhanced post-inoculation leaf peroxidase (POD) activity. In conclusion, DADS may enhance tobacco resistance to bacterial wilt by modulating the temporal expression of key NtPRX genes and activating antioxidant and defense responses. This study provides a molecular basis for understanding DADS-induced plant disease resistance.
陈佳丽, 邓文琦, 杨帆. 烟草PRX基因家族鉴定及其在DADS处理下响应青枯菌侵染的表达分析[J]. 农业生物技术学报, 2026, 34(9): 1901-1912.
CHEN Jia-Li, DENG Wen-Qi, YANG Fan. Identification and Expression Analysis of the PRX Gene Family in Tobacco (Nicotiana tabacum) in Response to Ralstonia solanacearum Infection Under DADS Treatment. 农业生物技术学报, 2026, 34(9): 1901-1912.
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