Genome-wide Identification of PHB Gene Family in Wheat (Triticum aestivum) and Its Expression Analysis in Response to High-temperature Stress
AN Gui1, JIN Han-Bing2, WANG Shu-Ping2,*, YE Shi1,*
1 Liangzhou District Seed Industry Center, Wuwei 733000, China; 2 College of Agriculture, Yangtze University/Key Laboratory of Green and Efficient Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Jingzhou 434025, China
Abstract：The prohibitins (PHB) are proteins characterized by SPFH domain, which play an important role in regulating plant development and senescence, and responding to biotic and abiotic stresses. High-temperature stress is one of the main abiotic stresses limiting wheat (Triticum aestivum) growth and yield increase. To identify key genes contributing to high-temperature stress in wheat, in this study, a comprehensive genome-wide analysis of the PHB gene family was performed. Using bioinformatics methods, the physiochemical properties, phylogenetic evolution, chromosome localization, collinearity and cis-elements in promoter sequences were all systematically analyzed in this study. Combined with transcriptome sequencing data and qRT-PCR, the expression patterns of PHB gene family members under high-temperature stress were also studied. In total, 61 TaPHB genes were identified in wheat genome, and all of them contained conserved PHB motifs. Subcellular localization prediction results suggested that most of the PHB gene members were localized in cytoplasm, others localized in mitochondria, chloroplasts and cell membranes, respectively. The phylogenetic tree analysis suggested that the PHB gene family could be classified into 5 clades, which were unevenly distributed on 21 chromosomes of wheat. Collinear analysis revealed that there were collinear relationships among a total of 44 gene pairs in wheat; In promoter regions of the PHB gene family, cis-acting elements involved in hormone regulation, light and abiotic stress response were identified. Combined transcriptome data analysis and qRT-PCR, 5 PHB gene members responding to high-temperature stress in leaves and roots of wheat were identified, which might play an important role in regulating wheat resistant to high-temperature stress. This study provides a theoretical basis for the in-depth analysis of the functions of PHB gene family members in wheat's response to high-temperature stress.
安贵, 金汉炳, 王书平, 叶石. 小麦TaPHB基因家族全基因组鉴定及其响应高温胁迫的表达分析[J]. 农业生物技术学报, 2026, 34(3): 610-626.
AN Gui, JIN Han-Bing, WANG Shu-Ping, YE Shi. Genome-wide Identification of PHB Gene Family in Wheat (Triticum aestivum) and Its Expression Analysis in Response to High-temperature Stress. 农业生物技术学报, 2026, 34(3): 610-626.
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