Construction of the Transient Overexpression System in Eggplant (Solanum melongena) Leaves
WANG Peng-Fei1,2, WANG Wu-Hong2, HU Hai-Jiao2, HU Tian-Hua2, WEI Qing-Zhen2, WANG Jing-Lei2, BAO Chong-Lai2, YAN Ya-Qin2,*
1 School of Life Sciences, Zhejiang Normal University, Jinhua 321000, China; 2 Vegetable Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China
Abstract：Plant transient expression technology serves as a key tool for rapid gene function identification and is widely applied in the optimization of plant genetic transformation systems and functional gene analysis. In this study, using eggplant (Solanum melongena) germplasm 'HQ1315' as the material, overexpression vector pCNG-Smechr0901901 carrying GFP reporter gene was constructed. Transient transformation efficiency was assessed through quantitative analysis of fluorescence intensity (using ImageJ software) combined with qRT-PCR technology. Key parameters, including Agrobacterium rhizogenes strains, inoculation methods, bacterial concentration (OD600), seedling age, vacuum pressure, treatment time, and acetosyringone (AS) concentration, were systematically optimized. The results demonstrated that the gene delivery efficiency of A. tumefaciens strain C58C1 in eggplant leaves was significantly higher than that of strains GV3101 and LBA4404 (P<0.05). Employing C58C1 as the mediating strain, with an AS concentration of 200 μmol/L and a bacterial suspension OD600 of 1.0, optimal transient overexpression efficiency was achieved by vacuum infiltration treatment of true-leaf stage eggplant leaves for 3 min under a vacuum pressure of -0.08 MPa. Furthermore, the eggplant leaf transient overexpression system established in this study achieved efficient and stable expression of the target gene in 4 representative eggplant varieties, namely Lvqie, Xianqie, Sanyueqie and Yuanqie. This study successfully established a highly efficient transient overexpression system for eggplant leaves, providing crucial technical support for subsequent functional analysis of disease resistance genes in eggplant.
王朋飞, 王五宏, 胡海娇, 胡天华, 魏庆镇, 汪精磊, 包崇来, 严亚琴. 茄子叶片瞬时过表达体系的构建[J]. 农业生物技术学报, 2026, 34(6): 1310-1321.
WANG Peng-Fei, WANG Wu-Hong, HU Hai-Jiao, HU Tian-Hua, WEI Qing-Zhen, WANG Jing-Lei, BAO Chong-Lai, YAN Ya-Qin. Construction of the Transient Overexpression System in Eggplant (Solanum melongena) Leaves. 农业生物技术学报, 2026, 34(6): 1310-1321.
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