Abstract：Establishment of plant regeneration system is essential for plant genetic transformation, and the use of antibiotics in plant genetic transformation systems is detrimental to cell differentiation, thus exacerbating the difficulty of establishing plant regeneration systems. Overexpression of the DELLA protein GbGAI4 (GAI: gibberellic acid insensitive) gene confers plant dwarfism, which is a visible and easily recognizable morphological phenotype in plants, and is expected to be used as a morphological screening marker in plant genetic transformation systems. In this study, pTA7002-GbGAI4 vector was constructed, the embryo tips of island cotton (Gossypium barbadense) 'Xinhai16' was infiltrated by Agrobacterium-mediated method, the expression of GbGAI4 gene was induced by dexamethasone, and then preliminarily screened out the transformed plants with dwarf characters, and further identified the transgenic plants with RT-PCR technology, so as to establish a noval genetic transformation system in cotton. Based on this, the growth-regulating factors (GRF) GRF4 was selected as the example gene for transgenic transformation based on the GbGAI4-mediated genetic transformation system, and a gene transformation rate of (4±0.6)% was obtained. This study provides a new genetic transformation platform for molecular breeding of cotton.
姑扎丽努尔·吐鲁洪, 张佳伟, 李悦, 王晓云, 张霞. DELLA蛋白介导的海岛棉新型遗传转化体系的建立[J]. 农业生物技术学报, 2024, 32(11): 2681-2690.
Guzhalinuer•TULUHONG, ZHANG Jia-Wei, LI Yue, WANG Xiao-Yun, ZHANG Xia. Establishment of a Novel DELLA Protein-mediated Genetic Transformation System in Gossypium barbadense. 农业生物技术学报, 2024, 32(11): 2681-2690.
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