Research Progress in Biological Characterisation and Inhibitory Mechanism of Microcin J25
LIU Yan1, GUO Gan-Tong1, HE Tao1, ZHANG Fan1, WANG Ya-Jie1, WU Jin-Mei2, QU Dong-Jing1,*
1 Sinagri YingTai Bio-peptide Co., Ltd., Zhengzhou 450000, China; 2 College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450000, China
Abstract：Microcin J25 (MccJ25) is a lasso-type antimicrobial peptide (AMP) derived from Escherichia coli, distinguished by its broad-spectrum antibacterial activity, low cytotoxicity, and negligible propensity to elicit bacterial drug resistance. Its unique lasso topology endows Mcc J25 with exceptional stability, rendering it highly resistant to harsh environmental conditions. Mcc J25 exerts bacteriostatic or bactericidal effects on target pathogens by inhibiting bacterial RNA polymerase activity and disrupting the membrane respiratory chain. Furthermore, this peptide mediates anti-inflammatory activity through the modulation of host immune responses and intestinal microecological homeostasis. Leveraging its pleiotropic anti-inflammatory mechanisms and synergistic antibacterial efficacy, Mcc J25 has emerged as a prime candidate for the development of next-generation antimicrobial agents. Herein, current research advances were synthesized to summarize the core biological properties and primary antibacterial pathways of Mcc J25, and its translational potential was further evaluated based on these characteristics, with the aim of providing a theoretical framework for future antimicrobial drug discovery and development.
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