Research Progress on the Application of Gene-editing Technology in Pig (Sus scrofa) Breeding for Disease Resistance
YAN Ying*, ZHAO Wu-Di*, ZHU Xiang-Xing**, TANG Dong-Sheng**
Guangdong Key Laboratory of Animal Molecular Design and Precision Breeding/Guangdong Gene Editing Engineering Technology Research Center, Foshan University, Foshan 528225, China
Abstract：As the world's largest producer and consumer of pork, the high-quality development of the pig (Sus scrofa) industry in China is directly related to the country's food safety and economic security. In recent years, outbreaks of African swine fever, Porcine reproductive and respiratory syndrome and Porcine epidemic diarrhea have caused significant economic losses to the pig farming industry. Traditional prevention and control methods face challenges such as lagging vaccine development and drug residues. Traditional disease resistant breeding has a long cycle and slow progress, and there is an urgent need to develop safer and more effective breeding technologies. The emerging gene editing technology, with advantages such as high efficiency, precision, and convenience, has shown great potential in agricultural animal breeding, including pigs, in recent years, providing strong technical support for the cultivation of new disease resistant pig breeds. This article systematically reviews the development history of gene editing technology, with a focus on analyzing the research progress of CRISPR/Cas9 and its derivative technologies in pig disease resistant breeding, aiming to provide theoretical basis and technical guidance for gene editing disease resistant pig breeding research.
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