Cloning of the Trypsin Gene in Larimichthys polyactis and Its Response to High Temperature Stress and Pseudomonas plecoglossicida Infection
LIU Hao-Wen1, LIU Feng2,*, ZHANG Tian-Le2, LI Qian3, LIU Si-Fang1, ZHU Jia-Jie4, LOU Bao2,*, YU Xiao-Ping1
1 College of Life Sciences, China Jiliang University, Hangzhou 310018, China; 2 Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences / Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Hangzhou 310021, China; 3 National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China; 4 School of Marine Sciences, Ningbo University, Ningbo 315832, China
Abstract：The frequent occurrence of summer heatwaves and visceral white-nodules disease caused by Pseudomonas plecoglossicida has emerged as critical constraints on the sustainable development of small yellow croaker (Larimichthys polyactis) aquaculture. Notably, trypsin, a key enzyme in amino acid metabolism, may play a pivotal role in mediating the physiological responses of small yellow croaker to both thermal stress and bacterial infection. To investigate the response characteristics of the trypsin gene in L. polyactis under high temperature stress and P. plecoglossicida infection, the CDS sequence of the trypsin gene (Lp_try) in L. polyactis was cloned in this study. The sequence of Lp_try spaned 744 bp, encoding 247 amino acids and containing the conserved Tryp_SPc domain. Expression analysis revealed that the Lp_try gene showed pan-tissue expression characteristics, but it showed a specific high expression pattern in the digestive system (intestine, liver) and circulatory system (heart). The expression characteristics of Lp_try gene in the liver of L. polyactis subjected to 32 ℃ high temperature stress and P. plecoglossicida infection were analyzed. The results showed that high temperature stress initially induced a significant increase of Lp_try expression (P<0.05), followed by a swift decreased with prolonged exposure. Additionally, during P. plecoglossicida infection, Lp_try expression exhibited a dynamic trend of decrease-increase-decrease over time, suggesting its regulatory role in the processes of L. polyactis in response to both high temperature stress and bacterial infection. This study provides an important foundation for further understanding of the physiological regulatory mechanisms in fish in response to high temperature stress and pathogen infection.
刘浩文, 刘峰, 张天乐, 李倩, 刘四芳, 朱家杰, 楼宝, 俞晓平. 小黄鱼Trypsin基因克隆及其对高温胁迫和变形假单胞菌感染的响应[J]. 农业生物技术学报, 2025, 33(5): 1106-1116.
LIU Hao-Wen, LIU Feng, ZHANG Tian-Le, LI Qian, LIU Si-Fang, ZHU Jia-Jie, LOU Bao, YU Xiao-Ping. Cloning of the Trypsin Gene in Larimichthys polyactis and Its Response to High Temperature Stress and Pseudomonas plecoglossicida Infection. 农业生物技术学报, 2025, 33(5): 1106-1116.
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