Analysis of Biofilm Formation Ability and Influencing Factors of Yak (Bos grunniens) Pathogenic Escherichia coli
GAO Xiang1,*, LI Hui-Ping1, LANG Xiao1, HE Zi-Wen1, WANG Jing-Hong1, WANG Xia1, NING Jian-Gang2,*
1 College of Biological Engineering and Technology, Tianshui Normal University, Tianshui 741001, China; 2 Gansu Province Tianzhu Tibetan Autonomous County Agriculture and Rural Bureau, Tianzhu 733299, China
Abstract：Biofilms exist widely in the natural environment. As one of the important anti-stress mechanisms of bacteria, they have strong resistance, pathogenicity and immune escape functions. The aim of this study was to establish a biofilm-forming model strain of yak (Bos grunniens) pathogenic Escherichia coli (YPEC), and to explore the effects of various boundary factors on the biofilm formation of YPEC. In this study, the isolates strains of YPEC Tianzhu-1, Tianzhu-2, Tianzhu-3, Qinghai-2, Qinghai-3 and Qinghai-4 were selected as the research object. Microplate quantative method was used to identify the biofilm formation ability of YPEC, and strains with strong biofilm formation were selected as the dominant strains, and then the influencing factors of biofilm formation amount were analyzed. The results showed that different isolates of YPEC had different biofilm forming abilities. Tianzhu-1, Tianzhu-2, and Tianzhu-3 strains were all strong biofilm formers, while Qinghai-3 and Qinghai-4 strains were medium biofilm formers, and Qinghai-2 strain were weak biofilm formers. Among them, Tianzhu-3 strain had the strongest ability to form biofilms (P<0.05). The optimal amount of biofilm formation of Tianzhu-3 strains was achieved when culture time was 24 h, temperature was 37 ℃, pH 7.5, initial bacterial solution concentration was 1.3×109 CFU/mL and 10% bacterial liquid ratio. The addition of 20 μg/mL DNA and 2% sucrose in LB medium could promote the formation of biofilm, while the addition of different concentrations of NaCl, glucose, and lactose all had inhibitory effects on the formation of biofilms. The above results provide data support and theoretical basis for YPEC prevention and control from the perspective of biofilm.
高翔, 黎会苹, 郎啸, 贺子文, 王婧虹, 王霞, 宁建刚. 牦牛致病性大肠杆菌生物被膜形成能力及影响因素分析[J]. 农业生物技术学报, 2024, 32(4): 873-881.
GAO Xiang, LI Hui-Ping, LANG Xiao, HE Zi-Wen, WANG Jing-Hong, WANG Xia, NING Jian-Gang. Analysis of Biofilm Formation Ability and Influencing Factors of Yak (Bos grunniens) Pathogenic Escherichia coli. 农业生物技术学报, 2024, 32(4): 873-881.
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