Abstract：This study was aim to analyze the testis tissue from healthy adult male ducks (Anas platyrhynchos) with differences in testicular size and semen production by RNA-seq and bioinformatics. The differentially expressed genes (DEGs) and signaling pathways related to testicular size and spermatogenesis were screened to provide data for supporting male duck breeding. In this study, 40 male ducks (230-day-old) were used to collect testes tissues according to testis size (light weight of testes, GS; heavy weight of testes, GL) and semen production (less semen volume, GF; excessive semen volume, GM). Collect the testicular tissues and use the BGIseq platform for transcriptomic mRNA sequencing, GO and KEGG functional annotation and enrichment analysis were used to analyze the candidate DEGs. The expression of DEGs were verified by qRT-PCR. The results showed that there was a significant difference in testicular size among male ducks with no significant difference in body weight, there was also a significant difference in the production of semen after artificial training. A total of 383 DEGs were analyzed in different semen production and different testis size groups, and 36 DEGs were screened among different groups. The results of qRT-PCR showed that the result of transcriptome sequencing was accurate and reliable. GO and KEGG analysis showed that DEGs was significantly enriched in signal transduction, cell membrane, membrane composition, plasma membrane, G protein-coupled receptor activity, positive regulation of cell division and cAMP signal, pI3K-AKT signal, calcium signal pathway and other related pathways, genes GPR50, GPR132, GRER1, IGF1, TACR3, HTR1F, FGF19, FGF3, GABRG1, ADCY1 were enriched in these pathways, which might be closely related to testicular function. This study screened the genes related different testicular size and spermatogenic capacity in ducks, which provides a theoretical basis for the genetic breeding of testicular function.
朱春红, 陶志云, 王志成, 宋卫涛, 刘宏祥, 章双杰, 徐文娟, 顾昊天, 王逸飞, 李慧芳. 健康成年公鸭睾丸大小比较及其相关调控基因表达分析[J]. 农业生物技术学报, 2024, 32(11): 2603-2613.
ZHU Chun-Hong, TAO Zhi-Yun, WANG Zhi-Cheng, SONG Wei-Tao, LIU Hong-Xiang, ZHANG Shuang-Jie, XU Wen-Juan, GU Hao-Tian, WANG Yi-Fei, LI Hui-Fang. Comparison of the Size of Testes in Healthy Adult Male Duck (Anas platyrhynchos) and Analysis of the Expression of Related Regulatory Genes. 农业生物技术学报, 2024, 32(11): 2603-2613.
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