Genetic Polymorphism of the TNNI3 and MOGAT2 Genes and Its Association Analysis with Fat Deposition Traits in Hu Sheep (Ovis aries)
ZHOU Ying-Hui1, WANG Wei-Min2, TIAN Hui-Bin2, XU Quan-Zhong2, MA Zong-Wu2, YANG Xiao-Bin2, ZHANG Jian1, CAO Pei-Liang1, CAI Tong1, MA Bo1, ZHANG Xiao-Xue1,*
1 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Grassland Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, China
Abstract：Troponin I3 (TNNI3), which regulates myocardial contraction by inhibiting actin-myosin interaction and serves as a core biomarker for the clinical diagnosis of myocardial infarction; monoacylglycerol O-acyltransferase 2 (MOGAT2) primarily catalyzes the synthesis of diacylglycerol from monoacylglycerol and fatty acids in the small intestine, playing a key role in dietary fat absorption and re-esterification. To investigate the association between SNP in the TNNI3 and MOGAT2 genes and fat deposition traits in Hu sheep (Ovis aries), a total of 1 029 male Hu sheep with clear pedigrees, complete phenotypic records, and healthy conditions were selected as experimental subjects in this study. Polymorphic loci of the TNNI3 and MOGAT2 genes were detected using PCR amplification and MassARRAY® SNP genotyping technology. A general linear model was used for association analysis of fat deposition-related traits, and the tissue expression patterns of the 2 genes were further determined. The results revealed 2 polymorphic sites: TNNI3 g.66954963 T>G, located in the first intron of TNNI3 gene, and MOGAT2 g.58004696 A>G, located in the second intron of MOGAT2 gene. Association analysis indicated that the TNNI3 g.66954963 T>G polymorphism was significantly associated with caudal fat weight, perirenal fat weight, and mesenteric fat weight (P<0.05). The MOGAT2 g.58004696 A>G polymorphism was significantly associated with perirenal fat weight, relative perirenal fat weight, mesenteric fat weight, and relative mesenteric fat weight (P<0.05). The combined genotype GGTNNI3-GGMOGAT2 showed significantly lower values for caudal fat weight, perirenal fat weight, relative perirenal fat weight, mesenteric fat weight, and relative mesenteric fat weight compared to the TTTNNI3-GAMOGAT2 genotype group (P<0.05). RT-qPCR analysis demonstrated that high expression of the TNNI3 gene in perirenal and mesenteric fat, while the MOGAT2 gene was highly expressed in caudal fat. In conclusion, the TNNI3 and MOGAT2 genes played important roles in fat deposition in Hu sheep. This study provides potential molecular markers for molecular-assisted breeding.
周颖慧, 王维民, 田慧彬, 徐全忠, 马宗武, 杨晓斌, 张健, 曹佩亮, 蔡桐, 马博, 张小雪. 湖羊TNNI3和MOGAT2基因遗传多态性与脂肪沉积性状的关联分析[J]. 农业生物技术学报, 2026, 34(6): 1232-1242.
ZHOU Ying-Hui, WANG Wei-Min, TIAN Hui-Bin, XU Quan-Zhong, MA Zong-Wu, YANG Xiao-Bin, ZHANG Jian, CAO Pei-Liang, CAI Tong, MA Bo, ZHANG Xiao-Xue. Genetic Polymorphism of the TNNI3 and MOGAT2 Genes and Its Association Analysis with Fat Deposition Traits in Hu Sheep (Ovis aries). 农业生物技术学报, 2026, 34(6): 1232-1242.
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