Identification of Biocontrol Bacillus QB-2-7 and BM-7 and Their Synthetic Microbial Community Effects on the Protection of Potato Anthracnose
LI Ya-Nan, ZHANG Hao-Jie, SHI Hui-Qin, LUO Ming-Kai, FENG Rui-Chao, YANG Qi-Lin, SHEN Shuo*
Academy of Agriculture and Forestry Sciences/Qinghai-Tibet Plateau Germplasm Resources Research and Utilization Laboratory/Key Laboratory of Qinghai-Tibetan Plateau Biotechnology of Ministry of Education/Key Laboratory of Potato Breeding of Qinghai/Engineering Research Center of Potato in Northwest Region Ministry of Education, Qinghai University, Xining 810016, China
Abstract：Potato anthracnose caused by Colletotrichum coccodes represents a significant phytopathological constraint in both potato (Solanum tuberosum) cultivation and postharvest storage. In this study, C. coccodes was used as the pathogen, and 2 endophytic bacteria (QB-2-7 and BM-7 ) with good bacteriostatic effect on C. coccodes preserved in the early stage of the laboratory were used to explore the control effect of its synthetic microbial community on potato anthracnose through in vivo and pot control experiments.Through comprehensive morphological characterization, physiological profiling, and molecular phylogenetic analysis, the isolates were taxonomically identified as Bacillus atrophaeus (QB-2-7) and B. safensis (BM-7), respectively. Biosafety assessment confirmed the non-hemolytic nature of both strains. Optimal consortium performance was achieved at a QB-2-7∶BM-7 ratio of 7∶3, demonstrating excellent microbial compatibility. Comparative analysis of different fermentation fractions revealed that the crude fermentation broth exhibited maximal antifungal activity (64.13% inhibition). In vivo evaluation of tuber protection efficacy demonstrated superior disease suppression in preventive applications (78.5% control efficiency) compared to both curative treatments and conventional azoxystrobin applications. The synthetic microbial community significantly enhanced host defense responses, as evidenced by elevated superoxide dismutase (SOD) and peroxidase (POD) activities, coupled with reduced polyphenol oxidase (PPO) activity and malondialdehyde (MDA) accumulation. Additional characterization revealed robust environmental tolerance of the fermentation products, including resistance to photodegradation, UV irradiation, and thermal stress, along with pronounced biofilm formation capacity. At the same time, the potato pot experiments showed that the synthetic microbial community had a significant prevention and control effect on potato anthracnose. These findings collectively suggest that the QB-2-7 and BM-7 synthetic microbial community consortium holds substantial promise as a biocontrol agent against potato anthracnose, providing both theoretical foundations and practical methodologies for developing microecological preparations targeting this economically important disease.
李亚楠, 张豪杰, 石晖琴, 罗明凯, 封瑞超, 杨麒麟, 沈硕. 马铃薯炭疽病生防芽胞杆菌QB-2-7和BM-7的鉴定及其合成菌群的生防作用[J]. 农业生物技术学报, 2025, 33(11): 2441-2459.
LI Ya-Nan, ZHANG Hao-Jie, SHI Hui-Qin, LUO Ming-Kai, FENG Rui-Chao, YANG Qi-Lin. Identification of Biocontrol Bacillus QB-2-7 and BM-7 and Their Synthetic Microbial Community Effects on the Protection of Potato Anthracnose. 农业生物技术学报, 2025, 33(11): 2441-2459.
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