1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2. State Key Laboratory for Animal Disease Control and Prevention/College of Veterinary Medicine of Lanzhou University/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730070, China
Abstract：Peste des petits ruminants virus (PPRV) infection induces diverse pathological damage in infected hosts and poses a serious threat to ruminant health. Transcriptome sequencing analysis of host cells following PPRV infection, conducted by our research group in the early stage, revealed a significant alteration in the expression level of the growth arrest and DNA damage-inducible 45A (GADD45A) gene. To investigate the potential role of GADD45A in PPRV replication, a recombinant lentiviral vector encoding the GADD45A gene was constructed, and a stable Vero cell line constitutively expressing GADD45A was established. qPCR, Western blot, and serial passage experiments confirmed sustained GADD45A expression at both mRNA and protein levels and demonstrated the stability of the cell line over multiple passages. The result showed that the established cell line maintained good viability and stable GADD45A expression throughout continuous passaging. The Vero cell line stably expressing GADD45A protein significantly enhanced the replication of PPRV. This study provides certain of biological tools and technical supports for further elucidating the molecular mechanisms underlying PPRV pathogenesis.
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