Production and Determination of Blood Physiology, Biochemistry and Growth Performance of Goats (Capra hircus) Overexpressing the NRAMP1 Gene
WANG Cong-Liang1*, CHEN Wen-Bo1*, WAN Shi-Cheng1, LI Jian-Nan1, REN Zhao-Fei2, HAN Bin3, LI Na1, LEI An-Min1, DU Xiao-Min2, QU Lei2, ZHU Hai-Jing2**, HUA Jin-Lian1**
1 Shaanxi Stem Cell Engineering and Technology Research Center, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; 2 Shaanxi Province Engineering & Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, China; 3 Yulin Animal Husbandry and Veterinary Service Center, Yulin 719000, China
Abstract：Natural resistance-associated macrophage protein 1 (NRAMP1) has been demonstrated to be linked with resistance to various intracellular mycobacterial diseases, including tuberculosis and brucellosis. This study aimed to develop goats (Capra hircus) with NRAMP1 gene overexpression and to conduct a comparative analysis of their blood physiology, biochemistry, and growth and development indices, which provides a research model and platform for establishing a brucellosis resistance model and for the genetic breeding of transgenic animals. Based on the lentiviral vector constructed in our laboratory for goat NRAMP1 gene, NRAMP1 gene overexpression goats were produced using the techniques of supernumerary ovulation and prokaryotic embryo microinjection, and subsequently monitored and evaluated the effects of this overexpression on physiological and biochemical changes in the blood, as well as on the growth and development of the goats. The results showed that the recovery rate of prokaryotic embryos was significantly higher than that observed at a dose of 270 IU for supernumerary ovulation when administering 210 IU follicle-stimulating hormone (FSH) in adult Shaanbei white cashmere goats (P<0.05). A total of 15 goats were obtained through the transplantation of prokaryotic embryos following microinjection, 3 of which were identified as positive gene overexpression goats via PCR, and then qRT-PCR and Western blot analyses revealed that NRAMP1 expression was significantly elevated at both the mRNA and protein levels in the testicular tissues of the NRAMP1 overexpression goats (P<0.05). The results of blood physiological and biochemical measurements indicated that there were no significant differences in blood physiological and biochemical indices between NRAMP1 overexpressing goats and wild-type goats (P>0.05). Similarly, the results of growth performance measurements revealed that the differences in growth traits, including newborn weight, body weight from 1~6 months of age, and body height and length from 3~6 months of age, between NRAMP1 gene overexpressing goats and wild-type goats were also not significant (P>0.05). In this study, goat model that overexpressing of the NRAMP1 gene was successfully developed, and notably, this overexpression did not adversely affect the health of the goats. This study provides a scientific basis for the breeding of brucellosis resistant goats and the study of their anti-resistance mechanism.
王聪亮, 陈文博, 万仕成, 李剑南, 任赵飞, 韩斌, 李娜, 雷安民, 杜晓敏, 屈雷, 朱海鲸, 华进联. NRAMP1基因过表达山羊的生产及血液生理生化和生长性能测定[J]. 农业生物技术学报, 2025, 33(4): 837-846.
WANG Cong-Liang, CHEN Wen-Bo, WAN Shi-Cheng, LI Jian-Nan, REN Zhao-Fei, HAN Bin, LI Na, LEI An-Min, DU Xiao-Min, QU Lei, ZHU Hai-Jing, HUA Jin-Lian. Production and Determination of Blood Physiology, Biochemistry and Growth Performance of Goats (Capra hircus) Overexpressing the NRAMP1 Gene. 农业生物技术学报, 2025, 33(4): 837-846.
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