Evaluation of the Oral Immune Effects of Pseudomonas plecoglossicida Immune Stimulating Complexes on Large Yellow Croaker (Larimichthys crocea)
XU Bin-Fu1, CHEN Xiu-Xia1, CHI Hong-Shu1, LI Su-Yi1, ZHENG Zai-Yu1, XU Meng-Ting1, JIANG Qiu-Huan1, ZHENG Wei-Qiang2, LIN Neng-Feng1,*
1 Fujian Provincial Aquatic Disease Prevention and Control Engineering Technology Research Center, Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 2 State Key Laboratory of Mariculture Breeding/Ningde FuFa Fisheries Company Limited, Ningde 352103, China
Abstract：Pseudomonas plecoglossicida is the main pathogen which causes the visceral white spot disease in large yellow croaker (Larimichthys crocea), leading to serious economic loss. Previous studies have shown that immune stimulating complexes (ISCOMs) have good oral immune effects. To evaluate the oral immune effects of P. plecoglossicida immune stimulating complexes (Pp-ISCOMs) on L. crocea, Pp-ISCOMs vaccine was prepared, which presented a cage-like nanoparticle structure. At 21 d post oral vaccination, the expression of immune-related genes in the head-kidney of immunized L. crocea were detected, and the immune serum titer was measured using agglutination tests. The immune protective effects of Pp-ISCOMs were then determined by challenge assay with P. plecoglossicida. The results showed that the expression of immune or anti-inflammatory factors such as myeloid differentiation factor 88 (MyD88)(P<0.05), interleukin 2 (IL-2)(P<0.001) in the head kidney of Pp-ISCOMs-immunized L. crocea were significantly higher than those of the inactived-P. plecoglossicida-immunized group (Pp) or the blank control group (CK); and the agglutination titer of large yellow croaker serum (21 d post immunization) was 22. After a P. plecoglossicida attack with a dose of 5×102 CFU/g by intraperitoneal injection, the relative percent survival (RPS) of L. crocea vaccinated by Pp-ISCOMs and Pp were estimated to be 73.3% and 40% respectively. The results indicate that the Pp-ISCOMs could improve the resistance of L. crocea to P. plecoglossicida, and suggested a promising prospect for application. This study provides technical support for the prevention and control by immune to visceral white spot disease in L. crocea.
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