Abstract:The new variety of large yellow croaker (Pseudosciaena crocea) named Donghai No.1 was selected from wild P. crocea which were captured from Daiquyang in Zhejiang Province in 2000. The group selection technology was performed by taking the traits of growth rate and low temperature tolerance as the selection index. The breeding line has got new aquatic variety certificate after the continuous five-generation mass selection in more than 10 years. Under the same cultured conditions, the body length and body weight of Donghai No.1 were 6.06% higher and 15.57% longer than those of commercial larvae at 19-month old. The survival rate of Donghai No.1 (10 month old) was 22.5% higher than that of commercial larvae at 6 ℃. During 2010 to 2012, the pilot scale of Donghai No.1 reached 70 000 m3 seawater, and the demonstration culture gained better social and economic benefits. The breeding process, germplasm characteristics, fine traits and pilot test of Donghai No.1 were described in this paper. This information will be helpful for the extension of Donghai No.1 and the breeding of other aquatic varieties.