|
|
|
| Qualitative and Quantitative PCR Detection of the Phytase(phyA2) Transgenic Corn |
| 1,1, 1, 1, 1, |
|
|
|
|
Abstract The detection of phytase transgenic corn is important to supervise its release and provides technical support. According to phytase gene (phyA2) sequences in transgenic phyA2 corn(Zea mays), the primers for qualitative and quantitative were designed. The qualitative PCR results showed that the primers were only effective to detect phyA2 transgenic corn, which indicated that the primers were highly specific. The SYBR Green I fluorescence quantitative PCR of endogenous reference zSSIIb zea mays starch synthase isoform zSTSII-2 and phyA2 genes were studied in this paper. Transgenic contens were calculated according to the standard Ct-copies linear graphs of the two genes. The results showed that the standard curves of zSSIIb and phyA2 genes had higher R2 value as 0.998 and 0.995, respectively, and the melting curves had single peak which mean the primers were specific. The mixed corn samples were detected to be 1.1%, 0.54% and 0.17%, respectively, which were close to the true values. The qualitative and quantitative PCR methods were developed in this study which can be effective at detecting phyA2 transgenic corn.
|
|
Received: 10 November 2011
|
|
|
|
|
|
|
