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Construction and Identification on cDNA Library of Breast
Muscle and Liver in Silkie Fowl |
Wang Xiuli1,3 Li Ning2** Zhao Xingbo1 Zhao Zhihui2 Deng Xuemei1
Li Changlü１ Qiu Xuemei3 Wu Changxin1 |
| ( 1. College of Animal Science and Technology, China Agricultural University, Beijing 100094,China; 2.State key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094,China；3. College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Mishan 158308,China) |
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Abstract Abstract: More and more animal breeding scientists are interested in chicken economic trait research. Most of these traits are related to different gene expression. So understanding the expressed genes and constructing the known gene expressed profile from muscle tissue and liver tissue of chicken are very important in animal breeding and genetics research. In order to prepare for the future comparative genomics research, functional genomics research, QTLs mapping, and animal molecular breeding etc, we have successfully constructed a breast muscle tissue cDNA libraries and a liver tissue cDNA libraries of Silkie Fowl (Gallus gallus ) in the study. The vector of cDNA used was Lambda ZAP II. At the same time, both cDNA libraries' quality were identified by the PCR for phage, the enzyme of EcoRⅠand XhoⅠ digested for plasmid and other methods. The results showed that the OD260/OD280 of mRNA from breast muscle and liver were 1.92 and 1.90 respectively, the titer of the breast library was 3.6×107 pfu/mL，the recombinant percentage was 92%，and the average length of inserted cDNA fragments was longer than1.5 kb. The titer of the liver cDNA library was 3.4×107 pfu/mL，the recombinant percentage was 90%，and the average length of inserted fragment cDNA was longer than 1.4 kb.
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Received: 01 January 1900
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