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| Comparative of Three Methods for the Induction of Tetraploidy Pearl Oyster (Pinctada martensi D.) |
| Wang Aimin 1,2 Yan Bing 2 Lan Guobao2 Ye Li 2 |
| （1. Fisheries Department, Hainan University,Haikou 570228，China； 2. Guangxi Institute of Oceanology, Beihai 536000，China ） |
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Abstract Abstract: Three methods for the induction of tetraploidy pearl oyster (Pinctada martensi D.) were compared. 1. Inhibiting release of the first polar body in fertilization of eggs from triploidy with sperm from diploidy resulted in the formation of tetraploid larvae at 27.7% ~ 35.9 %. But the death rate was high and no living tetraploid was detected after the larval stage. 2. Tetraploidy was induced by inhibition of the first and the second polar bodies in normal diploid zygotes. There were over 30% tetraploid in D-staged larva and the death rate was high. But at mid- oyster stage, i.e. 7 months cultivation, about 0.0625 % were tetraploid. 3. Inhibition of the first cleavage of normal diploid zygotes resulted in approximately 13.1% tetraploids at trochophore stage . However, the hatching rate was low and the death rate was high. Tetraploidy were not found after trochophore stage . The studies indicated that Inhibiting formation of the first and the second polar bodies in normal diploid zygotes could produce living tetraploid pearl oysters .
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Received: 01 January 1900
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