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| Improvement of a Method for Determining and Differentiating Virulence of Infectious Bursal Disease Virus (IBDV) Isolates and the Virulence Test of 7 Chinese IBDV Isolates |
Zhu Yiping1 Zhang Manfu1** Chen Guanchun2 Wang Xiaomei2 Wang Xiurong2 Thierry van den Berg 3
Dolores Morales3 Boonleong Lim4 |
| (1.College of Biological Sciences, China Agricultural University, Beijing 100094, China; 2. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China; 3. Section of Avian Virology and Biotechnology, Veterinary and Agrochemical Research Centre, 1180 Brussels, Belgium; 4. Department of Zoology, Hong Kong University, Hong Kong, China) |
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Abstract Abstract: A precise evaluation of virulence of infectious bursal disease virus (IBDV) is very important in studying the regulation of virulence variation and therefore is also important in finding the solutions to IBDV infection. However, the virulence determination method is not standardized. Normally, the virulence of IBDV is expressed as the mortality rate of SPF chicken or commercial chicken with variable inoculation dose, and the virus is titrated accordingly with TCID50、ELD50 or EID50 et al. The aim of the study was to unify IBDV titration route and standardize the method of IBDV virulence determination by greatly increasing the sensitivity of EID50 with chorioallantoic membrane (CAM) inoculation and high sensitive double-sandwich ELISA, hence made it possible to titrate all pathotypes of IBDV by EID50. The inoculation dose was 2×103 EID50 according to the survey report by Eterradossi. The standardized method was proved to be high sensitive, good reproducibility and universal adaptable by the virulence test of 7 Chinese isolates.
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Received: 01 January 1900
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