Identification of SPL Gene Family in Pinus massoniana and Their Expression in Response to Low Phosphorus Stress
WANG Chun-Yu1,2, FAN Fu-Hua1,2,*
1 Institute for Forest Resources and Environment of Guizhou/Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province, Guizhou University, Guiyang 550025, China; 2 College of Forestry, Guizhou University, Guiyang 550025, China
Abstract SPL (SQUAMOSA promoter binding protein (SBP)-like) is a plant specific transcription factor, and plays an important role in plant growth, development and metabolism. In this study, SPL family members were screened from the transcriptome data of Pinus massoniana obtained by the previous research. Through bioinformatics methods, the protein physical and chemical properties, subcellular localization prediction, conserved protein motif analysis, and phylogenetic tree analysis of PmSPL gene family were carried out. The results showed that 11 PmSPL family members were identified. All the family members were predicted to locate in the nucleus. The encoded proteins were composed of 190~1 147 amino acids, and the relative molecular mass were 21.6~127.1 kD. All the sequences contained motif1~3, which were conservative sequences of SBP domain. All the 11 PmSPLs contained 2 C2HC zinc finger structures and 1 nuclear localization signal (NLS). The response of PmSPL gene family to low phosphorus stress was detected by qRT-PCR. The results showed that the expression of PmSPL3 and PmSPL8 did not change significantly in the aboveground and underground parts of P. massoniana seedlings treated with low phosphorus at different times, which indicated that they did not respond to low phosphorus stress; PmSPL4 and PmSPL6 expression were significantly up-regulated in both aboveground and underground parts after 12 d of low phosphorus treatment, which might play a role in response to low phosphorus stress. This study provides a reference for further revealing the biological function of PmSPL gene family in response to low phosphorus stress.
WANG Chun-Yu,FAN Fu-Hua. Identification of SPL Gene Family in Pinus massoniana and Their Expression in Response to Low Phosphorus Stress[J]. 农业生物技术学报, 2023, 31(3): 509-517.
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