Cloning, Bioinformatics and Tissue Expression Profile Analysis of PDGFD Gene in Yak (Bos mutus)
Li Xin-Yi1,2, Wang Fu-Bin1,2, Huang Chun2, Ma Xiao-Ming2, La Yong-Fu2, Chu Min2, Guo Xian2, Yan Ping2, Pan He-Ping1, Liang Chun-Nian2,*
1 College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China; 2 Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
Abstract Yak (Bos mutus) is an important genetic resource to the plateau region. To study the structure and function of the platelet-derived growth factor D (PDGFD) gene in yaks, and explore the PDGFD gene expression in different tissues at 2 age stages, this study cloned the complete PDGFD gene CDS sequence by using subcutaneous adipose tissue cDNA of 36-month-old Datong yak as the template. Biological software was used for analyzing the PDGFD gene nucleotide sequence and physical and chemical properties of protein. The relative expression of PDGFD in 7 tissues (heart, liver, spleen, lungs, kidney, longissimus muscle and adipose tissue) of embryonic and 36-months old yak was carried out by qPCR. The results showed that the CDS region of PDGFD gene was 1 095bp and encoded 364 amino acids. The most closely genetic relationship of Datong yak was the B. mutus (99.8%), and the most distant was the Sus scrofa (91.2%). The protein analysis of PDGFD showed that PDGFD contains 1 CUB domain of 116 aa, 1 PDGF domain of 93 aa, and no transmembrane structure. PDGFD was predicted to be a stably hydrophilic secretory protein. The PDGFD protein advanced structure was consisted of random coil (48.21%), α-helix (25.62%), extended chain (21.49%), and β-turn (4.68%). In addition, PDGFD mainly interacted with platelet-derived growth factor protein and vascular endothelial growth factor protein. qPCR results showed that PDGFD mRNA was expressed in each tissue at 2 stages. By comparing the 2 periods, PDGFD gene expression was maintained at a high level in the heart. Meanwhile, the expression in adipose tissue changed the most. The mRNA expression level of PDGFD extremely significantly increased in adipose tissue with the growth and development of yaks (P<0.001). The result showed that PDGFD may be closely related to yak's heart, and affected adipose growth and development. This study offers a theoretical foundation for the regulation mechanism of yak fat deposition and provides references for the genetic breeding of yaks.
Li Xin-Yi,Wang Fu-Bin,Huang Chun, et al. Cloning, Bioinformatics and Tissue Expression Profile Analysis of PDGFD Gene in Yak (Bos mutus)[J]. 农业生物技术学报, 2023, 31(3): 518-529.
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