AbstractBegonia semperflorens are considered as an important garden ornamental plant, with its flowers open in clusters and gorgeous colors. In order to study the mechanism of the flower color formation of B. semperflorens, in this study, the anthocyanin synthesis pathway key enzyme flavonoid glycosyltransferase gene (UDP-glycose flavonoid glycosyltransferase, UFGT), were cloned and analyzed using B. semperflorens as materials, according to the previous stage of B. semperflorens transcriptome data. Differences in the expression of BsUFGT gene in different tissues of B. semperflorens were detected by fluorescence quantitative PCR and transformed into Nicotiana tabacum 'NC89' for functional verification. The results showed that a cDNA of 1 536 bp and 511 amino acids gene sequence was successfully cloned and named BsUFGT (GenBank No. OL329828). The molecular weight was 56 385.34 D, the theoretical isoelectric point (pI) was 5.25. Domain analysis showed that the UFGT protein encoded by BsUFGT gene had a UDPGT domain. Subcellular localization results showed that BsUFGT was mainly localized in cell membrane and nucleus.The qRT-PCR analysis showed that BsUFGT expression in B. semperflorens flowers was significantly higher than that in leaves and stems (P<0.05). BsUFGT of B. semperflorens were transformed into N. tabacum 'NC89' for functional verification. The anthocyanin content of anthocyanins in transgenic tobacco corolla was higher than that in wild-type tobacco corolla. This study provides a theoretical basis for the molecular regulation mechanism of anthocyanin synthesis in B. semperflorens.
CHEN Min,LIU Jing,LIU Zhi-Rou, et al. Cloning and Functional Verification of BsUFGT Gene in Begonia semperflorens[J]. 农业生物技术学报, 2023, 31(10): 2087-2097.
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