灰葡萄孢bZIP家族基因的全基因组鉴定与表达规律分析

doi:10.3969/j.issn.1674-7968.2022.04.014

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Abstract The bZIP (basic leucine zipper, bZIP) transcription factors are widely present in phytopathogenic fungi, and play an important regulatory role in the morphogenesis and infection of pathogens. Systematic studies of the bZIP transcription factor family in Botrytis cinerea have been rarely reported. In this study, the bZIP family genes in Botrytis cinerea were genome-wide identified, the protein physicochemical properties, phylogenetic evolution, conserved domain, and the expression pattern in pathogen conidia during development and infection period were analyzed. Meanwhile, qPCR was used to detect the expression of bZIP family genes after NaCl and H₂O₂ treatment. The results showed that there were 16 bZIP family genes identified from Botrytis cinerea genome, which were divided into 4 subfamilies by phylogenetic analysis. All bZIP family genes of Botrytis cinerea contained the typical BRLZ domain of bZIP family. Some genes showed high expression levels at different development of conidia and infection stages of Botrytis cinerea. All bZIP family genes of Botrytis cinerea were significantly down-regulated after NaCl and H₂O₂ treatment. These results indicated that bZIP family genes of Botrytis cinerea played an important role in the growth and development, infection process and response to salt stress and oxidative stress. This study provides a theoretical basis for further revealing the function and molecular mechanism of bZIP family genes of Botrytis cinerea.

Key words： Botrytis cinerea bZIP family gene Bioinformatics analysis Expression analysis

Received: 17 June 2021

ZTFLH:

S435.131

Corresponding Authors: *xingjihong2000@126.com; dongjingao@126.com

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	LI Bai
	ZHANG Zi-Yan
	ZHANG Qiang
	CAO Hong-Zhe
	ZANG Jin-Ping
	ZHANG Kang
	XING Ji-Hong
	DONG Jin-Gao

Cite this article:

LI Bai,ZHANG Zi-Yan,ZHANG Qiang, et al. Genome-wide Identification and Expression Analysis of bZIP Family Genes in Botrytis cinerea[J]. 农业生物技术学报, 2022, 30(4): 762-771.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2022.04.014 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2022/V30/I4/762

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