海岛棉<em>GbHCT13</em>基因的克隆及表达特性分析

doi:10.3969/j.issn.1674-7968.2021.06.003

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Abstract Shikimate quinate hydroxycinnamoyl transferase (HCT) is one of the key enzyme in lignin synthesis pathway, also affected the quality of cotton (Gossypium spp.) fiber. The study on the expression characteristics of GbHCT13 in cotton fiber development can provide a reference for exploring the mechanism of cotton fiber quality development in the future. HCT gene involved in lignin metabolism named GbHCT13 was cloned from the fiber (10 d) in Gossypium barbadense 'XH21'. Bioinformatics analysis was carried out on its sequence.The expression patterns and characteristics were analyzed in G. barbadense and G. hirsutum by qRT-PCR, respectively. Effects of caffeoyl coenzyme A on fiber development were studied by in vitro feeding experiment of cotton ovule. These results showed that GbHCT13 gene (GenBank No. MW048849) was located on the Gossypium barbadense chromosome 10 of group D, the total length was 1 400 bp, CDS coding region was 1 311 bp, predicted that the proteins encoded by this gene were a class of hydrophilic non-membrane proteins, consisting of 1~432 amino acids, and the multi-sequence comparison from other species revealed that the amino acid sequence of GbHCT13 contained a BAHD acyltransferase family of HXXXD and DFGWG regions, which were closely related to Gossypium arboreum and Gossypium raimondii. Using qRT-PCR analysis GbHCT13 gene expressed in different cotton varieties , the results showed that the gene expression in different Gossypium barbadense varieties were similar trends. The expression level was higher at 5~15 d in the early stage of fiber development, especially at the 10 d of fiber development. In vitro feeding of ovule showed that certain concentration of caffeoyl coenzyme A promoted fiber elongation and the expression of GbHCT13 gene. This study provides the theoretical basis for the future study of the molecular mechanism of GbHCT13 gene involved in lignin synthesis.

Key words： Gossypium barbadense GbHCT13 Ovule culturing in vitro Lignin

Received: 16 September 2020

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Corresponding Authors: * chqjia@126.com

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	TENG Lu
	ZHENG Kai
	QU Yan-Ying
	LEI Hui-Chen
	CAI Yong-Sheng
	CHEN Quan-Jia

Cite this article:

TENG Lu,ZHENG Kai,QU Yan-Ying, et al. Cloning and Expression Characteristics Analysis of GbHCT13 Gene in Gossypium barbadense[J]. 农业生物技术学报, 2021, 29(6): 1050-1060.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.06.003 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I6/1050

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