Expression of Cell Wall Metabolism Genes During Tomato (Solanum lycopersicum) Maturation and the Effects of Treatment with Exogenous C2H4 and ABA on Their Expression
ZENG Wen-Jing, YANG Jun, CHEN Shi-Mei-Jie, WEI Xiao-Ying, WEN Guo-Qin, HE Zhuo-Yuan, WANG Qian, CHEN Xi-Tong, ZHOU Jia-Yan, ZOU Jian*
Key laboratory of Southwest Wildlife Resources Protection (Ministry of Education), China West Normal University, Nanchong 637009, China
Abstract Firmness is an important indicator of fruit shelf-life, and cell wall metabolism is an important factor affecting the firmness and softening process of fruit. In order to understand the relationship among the softening process, cell wall metabolism genes and regulation of fruit ripening hormones in tomato (Solanum lycopersicum), the expression of cell wall metabolism genes during tomato fruit ripening was systematically analyzed, and the effects of the fruit ripening hormones, abscisic acid (ABA), ethylene (C2H4) and their inhibitors on the expression of these genes were investigated by applying exogenous hormones and their inhibitors and qRT-PCR technique. The results showed that the expression of cell wall metabolism related genes, SlCel1 (endo-β-1,4-glucanases), SlTBG7 (β-galactosidase precursor), SlXETB2-F2 (xyloglucanendotransglycosylase), SlXET4-F1, SlTBG3, SlCel2, SlExp1 (expansin), SlTBG1, SlPG (polygalacturonase) and SlTBG4 are increased with fruit maturation and softening, suggesting their close relation to the softening process during fruit ripening in tomato. The treatment results of fruit ripening hormones and their inhibitors indicated that exogenous ABA could promote the expressing of SlCel1, SlExp1, SlTBG3, SlTBG4 and SlXET4-F1 genes,while the expression of SlCel1, SlExp1 and SlTBG4 genes was inhibited by NDGA (nordihydroguaiaretic acid). The application of ethylene could up-regulate the expressing of SlExp1, SlPG and SlTBG4 genes, but their expressions were inhibited by 1-MCP (1-methylcyclopropene). These results implied that the regulation of cell wall metabolism related genes, SlCel1, SlExp1, SlTBG3, SlTBG4, SlPG and SlXET4-F1 require ABA and ethylene to regulate the softening process during fruit ripening. Therefore, appropriate application of NDGA or 1-MCP might be beneficial for inhibiting cell wall metabolism and would extend the fruit shelf-life by inhibiting the ABA and ethylene signaling. The cultivation of new tomato varieties with low expression levels of SlPG, SlCel1, SlExp1 and SlTBG4 genes may effectively realize a new way to keep tomatoes fresh and maintain good fruit quality after harvest. These results has guiding significance for realizing the ripening regulation of tomato fruit.
ZENG Wen-Jing,YANG Jun,CHEN Shi-Mei-Jie, et al. Expression of Cell Wall Metabolism Genes During Tomato (Solanum lycopersicum) Maturation and the Effects of Treatment with Exogenous C2H4 and ABA on Their Expression[J]. 农业生物技术学报, 2021, 29(6): 1040-1049.
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